Study type: Medical/biological study (experimental study)

Experimental model for ELF-EMF exposure: Concern for human health. med./bio.

Published in: Saudi J Biol Sci 2015; 22 (1): 75-84

Aim of study (acc. to author)

The effect of an in vitro exposure of four different cell lines to a 50 Hz magnetic field on the expression of MCP-1 should be investigated.

Background/further details

The monocyte chemoattractant protein-1 (MCP-1) is a cytokine which guides monocytes and other leukocytes to centers of inflammation and infection and has importance in diseases like rheumatism and atherosclerosis. The authors speculated that an anti-inflammatory effect of extremely low frequency magnetic fields described in literature could be caused by an alteration of the MCP-1 expression. THP-1 cells were also tested after a stimulation with lipopolysaccharides and K562 cells after a stimulation with phorbol myristate acetate.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 4-72 h

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 4-72 h
Exposure setup
Exposure source
Chamber cells were exposed inside an incubator in the central part of the solenoid
Setup solenoid placed inside the incubator; solenoid consisted of 160 turns of wire (22 cm length, 6 cm radius, copper wire diameter of 1.25 x 10-5 cm) generating a horizontal magnetic field; signal had a stability higher than 1% both in frequency and in amplitude and 98% homogeneity at location of cells; temperature remained at 37± 0.38°C
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT effective value measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

A significantly decreased MCP-1 gene expression and protein expression rate was found in exposed HaCaT cells compared to the control after 4 to 72 hours of exposure. No significant differences were observed between exposure and control in SH-SY5Y cultures after 24 hours of exposure (comment of EMF-Portal: contradictory results in the running text and in the table). No significant differences could be found in exposed THP-1 cells compared to the control, either. In the tests with K562 cells, a significant increase in MCP-1 expression was found in exposed cells compared to the control, when these were additionally treated with PMA, a stimulant for the differentiation into monocytes and megakaryocytes.
The authors conclude that an in vitro exposure of cells to a 50 Hz magnetic field could influence the MCP-1 expression cell specifically.

Study character:

Study funded by

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