研究のタイプ: 医学/生物学の研究 (experimental study)

[ELF-EMFばく露に関する実験用のモデル:人の健康への懸念] med./bio.

Experimental model for ELF-EMF exposure: Concern for human health.

掲載誌: Saudi J Biol Sci 2015; 22 (1): 75-84

この研究は、正弦波磁界EMF:50Hz、磁束密度実効値1mT)にばく露したヒト皮膚角化細胞HaCaT、ヒト神経芽腫細胞株SH-SY5Y、ヒト単球細胞株THP-1、ヒト白血球細胞株K562でのサイトカインMCP-1の発現を調べた。結果として、MCP-1の発現へのEMFの影響には細胞の種類による違いが見られた、と報告している。

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研究目的(著者による)

The effect of an in vitro exposure of four different cell lines to a 50 Hz magnetic field on the expression of MCP-1 should be investigated.

詳細情報

The monocyte chemoattractant protein-1 (MCP-1) is a cytokine which guides monocytes and other leukocytes to centers of inflammation and infection and has importance in diseases like rheumatism and atherosclerosis. The authors speculated that an anti-inflammatory effect of extremely low frequency magnetic fields described in literature could be caused by an alteration of the MCP-1 expression. THP-1 cells were also tested after a stimulation with lipopolysaccharides and K562 cells after a stimulation with phorbol myristate acetate.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 4-72 h

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 4-72 h
ばく露装置
ばく露の発生源/構造
チャンバの詳細 cells were exposed inside an incubator in the central part of the solenoid
ばく露装置の詳細 solenoid placed inside the incubator; solenoid consisted of 160 turns of wire (22 cm length, 6 cm radius, copper wire diameter of 1.25 x 10-5 cm) generating a horizontal magnetic field; signal had a stability higher than 1% both in frequency and in amplitude and 98% homogeneity at location of cells; temperature remained at 37± 0.38°C
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT effective value 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

A significantly decreased MCP-1 gene expression and protein expression rate was found in exposed HaCaT cells compared to the control after 4 to 72 hours of exposure. No significant differences were observed between exposure and control in SH-SY5Y cultures after 24 hours of exposure (comment of EMF-Portal: contradictory results in the running text and in the table). No significant differences could be found in exposed THP-1 cells compared to the control, either. In the tests with K562 cells, a significant increase in MCP-1 expression was found in exposed cells compared to the control, when these were additionally treated with PMA, a stimulant for the differentiation into monocytes and megakaryocytes.
The authors conclude that an in vitro exposure of cells to a 50 Hz magnetic field could influence the MCP-1 expression cell specifically.

研究の種別:

研究助成

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