この研究は、正弦波磁界（EMF：50Hz、磁束密度実効値1mT）にばく露したヒト皮膚角化細胞HaCaT、ヒト神経芽腫細胞株SH-SY5Y、ヒト単球系細胞株THP-1、ヒト白血球細胞株K562でのサイトカインMCP-1の発現を調べた。結果として、MCP-1の発現へのEMFの影響には細胞の種類による違いが見られた、と報告している。
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The effect of an in vitro exposure of four different cell lines to a 50 Hz magnetic field on the expression of MCP-1 should be investigated.
The monocyte chemoattractant protein-1 (MCP-1) is a cytokine which guides monocytes and other leukocytes to centers of inflammation and infection and has importance in diseases like rheumatism and atherosclerosis. The authors speculated that an anti-inflammatory effect of extremely low frequency magnetic fields described in literature could be caused by an alteration of the MCP-1 expression. THP-1 cells were also tested after a stimulation with lipopolysaccharides and K562 cells after a stimulation with phorbol myristate acetate.
| ばく露 | パラメータ |
|---|---|
|
ばく露1:
50 Hz
ばく露時間:
continuous for 4-72 h
|
|
| 周波数 | 50 Hz |
|---|---|
| タイプ |
|
| 波形 |
|
| ばく露時間 | continuous for 4-72 h |
| ばく露の発生源／構造 | |
|---|---|
| チャンバの詳細 | cells were exposed inside an incubator in the central part of the solenoid |
| ばく露装置の詳細 | solenoid placed inside the incubator; solenoid consisted of 160 turns of wire (22 cm length, 6 cm radius, copper wire diameter of 1.25 x 10-5 cm) generating a horizontal magnetic field; signal had a stability higher than 1% both in frequency and in amplitude and 98% homogeneity at location of cells; temperature remained at 37± 0.38°C |
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 磁束密度 | 1 mT | effective value | 測定値 | - | - |
A significantly decreased MCP-1 gene expression and protein expression rate was found in exposed HaCaT cells compared to the control after 4 to 72 hours of exposure. No significant differences were observed between exposure and control in SH-SY5Y cultures after 24 hours of exposure (comment of EMF-Portal: contradictory results in the running text and in the table). No significant differences could be found in exposed THP-1 cells compared to the control, either. In the tests with K562 cells, a significant increase in MCP-1 expression was found in exposed cells compared to the control, when these were additionally treated with PMA, a stimulant for the differentiation into monocytes and megakaryocytes.
The authors conclude that an in vitro exposure of cells to a 50 Hz magnetic field could influence the MCP-1 expression cell specifically.
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