Study type: Medical/biological study (experimental study)

Influence of Electromagnetic Fields on Lead Toxicity: A Study of Conformational Changes in Human Blood Proteins. med./bio.

Published in: Iran Red Crescent Med J 2016; 18 (7): e28050-

Aim of study (acc. to author)

The effects of co-exposure to a 50 Hz magnetic field and lead ions on oxidative stress in human blood should be investigated.

Background/further details

55 blood samples from age- and sex-matched healthy volunteers were used. The group division is contradictory. On the one hand, a division into 5 groups (n=11) is mentioned without further definition. On the other hand, the figures in the publication suggest the following grouping: 1) exposure to the magnetic field without lead supplement, 2) co-exposure to the magnetic field and 10 μM, 3) 20 μM, 4) 30 μM, 5) 40 μM, 6) 50 μM, 7) 60 μM, 8) 70 μM, 9) 80 μM, 10) 90 μM and 11) 100 μM lead chloride. For each of these groups, a separate control group was used which contained the same amount of lead chloride.
To determine the factors affecting the oxyhemoglobin, an artificial neural network analysis was applied.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: for 120 minutes

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration for 120 minutes
Exposure setup
Exposure source
Setup the signal generator was connected to coils; external diameter and width of each coil were 56 cm and 4.5 cm, respectively
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2 mT - - - -

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

(Remark EMF-Portal: The presentation and interpretation of the results are not comprehensible in a synopsis. It is not clear which groups the authors refer to in the results. The figures (see full text) show a general trend that the effect of the magnetic field decreased with increasing lead concentration, what is not mentioned or interpreted in the flow text. The results presented below refer to the flow text according to the authors.)
Blood samples from the magnetic field co-exposure groups showed significantly increased concentrations of hemoglobin, met-hemoglobin and hemichrome as well as a significantly reduced interaction of the globin chain and heme ring and a significantly reduced heme-heme interaction compared to the control groups (i.e. only lead). This indicated the conversion of oxyhemoglobin to met-hemoglobin in the magnetic field co-exposure groups, showing oxidative damage of the erythrocytes.
The content of carbonyl proteins increased significantly in the magnetic field co-exposure groups with increasing lead concentration. Significant positive correlations were found between the carbonyl protein content and the hemoglobin and met-hemoglobin content in the magnetic field co-exposure groups.
The artificial neural network analysis showed the significant importance of hemichromes, met-hemoglobin and the lead concentration in the blood for the oxyhemoglobin level.
The authors conclude that exposure to a 50 Hz magnetic field could increase the oxidative stress caused by lead ions in human blood.

Study character:

Study funded by

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