研究のタイプ: 医学/生物学の研究 (experimental study)

[鉛毒性に対する電磁界の影響:ヒト血液タンパク質での立体構造変化の研究] med./bio.

Influence of Electromagnetic Fields on Lead Toxicity: A Study of Conformational Changes in Human Blood Proteins.

掲載誌: Iran Red Crescent Med J 2016; 18 (7): e28050-

この研究は、ヒト血液ヘモグロビン(Hb)および血漿タンパク酸化的変化に対する鉛イオン(投与濃度:0-100μMの11段階)と電磁界EMF:2mT、50Hz、120分ばく露)の相乗的影響の有無を調べた。健康被験者から採血した血液プールした後、55個の標本に分けた。それぞれに各濃度の鉛を投与した後、ヘルムホルツコイルを用いてEMFの120分ばく露を実施し、無ばく露対照群と比較した。その結果として、共ばく露によるメトヘモグロビン(metHb)濃度、ヘミクロム濃度の変化など、多くの血液パラメータに関するデータを示し、EMF存在下の鉛混合血液では酸化損傷が増強されると報告している。

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研究目的(著者による)

The effects of co-exposure to a 50 Hz magnetic field and lead ions on oxidative stress in human blood should be investigated.

詳細情報

55 blood samples from age- and sex-matched healthy volunteers were used. The group division is contradictory. On the one hand, a division into 5 groups (n=11) is mentioned without further definition. On the other hand, the figures in the publication suggest the following grouping: 1) exposure to the magnetic field without lead supplement, 2) co-exposure to the magnetic field and 10 μM, 3) 20 μM, 4) 30 μM, 5) 40 μM, 6) 50 μM, 7) 60 μM, 8) 70 μM, 9) 80 μM, 10) 90 μM and 11) 100 μM lead chloride. For each of these groups, a separate control group was used which contained the same amount of lead chloride.
To determine the factors affecting the oxyhemoglobin, an artificial neural network analysis was applied.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露装置
ばく露の発生源/構造
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2 mT - - - -

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

(Remark EMF-Portal: The presentation and interpretation of the results are not comprehensible in a synopsis. It is not clear which groups the authors refer to in the results. The figures (see full text) show a general trend that the effect of the magnetic field decreased with increasing lead concentration, what is not mentioned or interpreted in the flow text. The results presented below refer to the flow text according to the authors.)
Blood samples from the magnetic field co-exposure groups showed significantly increased concentrations of hemoglobin, met-hemoglobin and hemichrome as well as a significantly reduced interaction of the globin chain and heme ring and a significantly reduced heme-heme interaction compared to the control groups (i.e. only lead). This indicated the conversion of oxyhemoglobin to met-hemoglobin in the magnetic field co-exposure groups, showing oxidative damage of the erythrocytes.
The content of carbonyl proteins increased significantly in the magnetic field co-exposure groups with increasing lead concentration. Significant positive correlations were found between the carbonyl protein content and the hemoglobin and met-hemoglobin content in the magnetic field co-exposure groups.
The artificial neural network analysis showed the significant importance of hemichromes, met-hemoglobin and the lead concentration in the blood for the oxyhemoglobin level.
The authors conclude that exposure to a 50 Hz magnetic field could increase the oxidative stress caused by lead ions in human blood.

研究の種別:

研究助成

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