研究のタイプ: 医学/生物学の研究 (experimental study)

[虚血性脳卒中における低周波数パルス電磁界の神経保護効果] med./bio.

Neuroprotective Effect of Low Frequency-Pulsed Electromagnetic Fields in Ischemic Stroke.

掲載誌: Appl Biochem Biotechnol 2017; 181 (4): 1360-1371

この研究は、虚血脳卒中後の神経保護に対する低周波パルス電磁界LF-PEMF)の影響を調べるため、光血栓性閉塞後のC57B6系統のマウスを60Hz、10mTのLF-PEMFにばく露した。LF-PEMF処理群および非処理群の両方の虚血脳卒中マウスで、BDNF/TrkB/Aktシグナル伝達経路アポトーシス促進性および生存促進性のタンパク質および遺伝子発現炎症性メディエーターを測定し、行動学的検査を実施した。その結果、LF-PEMF処理はBDNF/TrkB/Aktシグナル伝達経路の活性化を促進した。その後、LF-PEMF処理後の虚血脳卒中マウスで、生存促進タンパク質が有意に増加したが、アポトーシス促進タンパク質及び炎症性メディエーターは減少した。著者らは、この結果はLF-PEMFばく露には虚血脳卒中後のマウスの回復過程における神経保護効果があることを証明した、と結論付けている。

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研究目的(著者による)

The neuroprotective effects and mechanisms of action of exposure of mice to a pulsed 60 Hz magnetic field after ischemic stroke should be investigated.

詳細情報

Ischemic strokes were experimentally induced in male mice. After the stroke, mice were divided into 2 groups (n=18 each): 1) exposure to the magnetic field and 2) sham exposure. Mice from each group were killed after 1, 3 and 14 days of exposure (remark EMF-Portal: respective numbers were not stated).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 60 Hz
ばく露時間: intermittent for 6 h/day for up to 14 days

General information

exposure started within 30-40 min after experimentally induced stroke

ばく露1

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
波形
  • pulsed
ばく露時間 intermittent for 6 h/day for up to 14 days
Additional information no details on pulse modulation
ばく露装置
ばく露の発生源/構造
  • 詳細不明
ばく露装置の詳細 mice could move freely during exposure
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 10 mT - 計算値 - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露前
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

Exposed mice had significantly longer rotarod times compared to sham exposed mice, indicating better motoric functions.
Previous studies suggested that BDNF and protein kinase C play a central role in neuroprotection, which was confirmed in this study by a significantly increased expression of BDNF, TrkB and phosphorylated protein kinase B in exposed mice compared to sham exposed mice after 3 and 14 days. This resulted further in a significant decrease of gene expression and protein expression of pro-apoptotic Bad, Bax, caspase 3 and a significant increase of anti-apoptotic Bcl-xL expression in the exposure group compared to the sham exposure group.
Moreover, the protein expression of inflammatory mediators MMP9 and IL-1β was significantly decreased in exposed mice compared to sham exposed mice after 3 and 14 days of exposure.
The authors conclude that exposure of mice to a pulsed 60 Hz magnetic field might have a neuroprotective effect after ischemic stroke via suppression of inflammation and apoptosis. They propose that low frequency pulsed electromagnetic fields may be beneficial as a treatment during recovery after ischemic stroke.

研究の種別:

研究助成

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