The aim of the study was to find a better definition of the calcium sites responding to weak electrical stimulation.
First, changes in calcium efflux were studied in relation to calcium concentration in the medium, because calcium-calcium interactions have often been shown to play an important role in the regulation of calcium uptake and release.
Another approach to differentiating possible components of the 45Ca2+ efflux was to attempt modification of calcium release by adding lanthanum. The trivalent lanthanum ion has been found to be effective in blocking calcium flux across excitable membranes.
16 Hz AM modulated radiofrequency is capable to stimulate the calcium efflux of pre-incubated isolated chicken brain tissue. Calcium concentration (in the range of 0 - 4.16 mM) in the bathing solution had no significantly effect on calcium efflux, compared with control. Calcium efflux was inhanced by the addition of H+ (HCl (0.108 mM)) and inhibited by the absence of normal bicarbonate levels (2.4 mM HCO3-). The addition of lanthanum (0.5 mM LaCl3) to bicarbonate-free solutions leads to an decreased calcium efflux in irradiated samples.