Study type: Medical/biological study (experimental study)

Microwave radiation-induced calcium ion efflux from human neuroblastoma cells in culture. med./bio.

Published in: Bioelectromagnetics 1984; 5 (1): 71-78

Aim of study (acc. to author)

To investigate whether the in vitro exposure to 915 MHz microwaves irradiation exhibits effects on the calcium flux (influx/efflux) in human brain tissue.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 915 MHz
Modulation type: AM
Exposure duration: continuous for 30 min
  • SAR: 5 mW/g maximum (0.01, 0.05, 0.075, 0.1, 0.5, 0.75, 1.0, 1.5, 2.0, or 5.0 mW/g)
Exposure 2: 915 MHz
Modulation type: AM
Exposure duration: continuous for 30 min
  • SAR: 0.05 mW/g unspecified
Exposure 3: 915 MHz
Modulation type: CW
Exposure duration: continuous for 30 min
  • SAR: 0.05 mW/g unspecified
  • SAR: 1 mW/g unspecified

Exposure 1

Main characteristics
Frequency 915 MHz
Type
Charakteristic
  • guided field
Exposure duration continuous for 30 min
Additional info Dutta SK, Choppalla J, Hossain MA, Bhar TN, Ho Hs (1982): Dosimetric measurement and bioeffect studies of low level 915 MHz CW microwaves using TEM Crawford cell. J Basic Appl Sci 2: 43-52.
Modulation
Modulation type AM
Modulation frequency 16 Hz
Modulation depth 80 %
Additional info

sinusoidal

Exposure setup
Exposure source
Chamber The exposure system was basically similar to that described by Blackman et al. [1979]. The Crawford cell consisted of a rectangular TEM transmission line tapered at each end to mate with a coaxial cable. It was placed in an incubator kept at 37°C.
Setup One tissue culture flask (T-flask) of 25 cm² growth surface was placed on either side of the center plane in the TEM cell.
Sham exposure A sham exposure was conducted.
Additional info For sham exposure, flasks were placed inside the exposure chamber and treated for the same time without microwaves. Control flasks were kept outside the exposure chamber in the same incubator.
Parameters
Measurand Value Type Method Mass Remarks
SAR 5 mW/g maximum determined by power loss - 0.01, 0.05, 0.075, 0.1, 0.5, 0.75, 1.0, 1.5, 2.0, or 5.0 mW/g

Exposure 2

Main characteristics
Frequency 915 MHz
Type
Charakteristic
  • guided field
Exposure duration continuous for 30 min
Modulation
Modulation type AM
Modulation frequency 3–30 Hz
Additional info

sinusoidal

Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
SAR 0.05 mW/g unspecified determined by power loss - -

Exposure 3

Main characteristics
Frequency 915 MHz
Type
Charakteristic
  • guided field
Exposure duration continuous for 30 min
Modulation
Modulation type CW
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
SAR 0.05 mW/g unspecified determined by power loss - -
SAR 1 mW/g unspecified determined by power loss - -

Reference articles

  • Blackman CF et al. (1979): Induction of calcium-ion efflux from brain tissue by radio frequency radiation: Effects of modulation frequency and field strength.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • before exposure
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

A significant increase in the calcium efflux occurred at two specific absorption rate values: 0.05 and 1 mW/g. The increased calcium efflux at the specific absorption rate of 0.05 mW/g was dependent on the presence of amplitude modulation at 16 Hz but at the higher value of 1 mW/g specific absorption rate it was not observed.

Study character:

Study funded by

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