Study type: Medical/biological study (experimental study)

S1P mediates human amniotic cells proliferation induced by a 50-Hz magnetic field exposure via ERK1/2 signaling pathway. med./bio.

Published in: J Cell Physiol 2019; 234 (6): 7734-7741

Aim of study (acc. to author)

The possible role of sphingosine‐1‐phosphate in 50‐Hz magnetic fieldinduced cell proliferation of human amniotic cells should be investigated.

Background/further details

Cells were either exposed or sham exposed. In some tests, cells were tested under addition of SKI II, an inhibitor of sphingosine kinase, or U0126, an inhibitor of ERK 1 and 2.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 60 min

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 60 min
Exposure setup
Exposure source
Chamber two exposure chambers which were put in a CO2 incubator
Setup one chamber was used for exposure and the other for sham exposure; each chamber consisted of a set of square Helmholtz coils (20 × 20 cm2), which was encased by mu‐metal for shielding the cells placed in the coils from stray magnetic fields; a fan on the metal wall ensured the air and the temperature was uniform between inside the chamber and incubator; during exposure, cell dishes were put in the center of the chambers; the temperature in the chambers was kept at 37.0 ± 0.2°C throughout the whole exposure period; the difference of the temperature between exposure and sham exposure conditions during the entire exposure period did not exceed 0.1°C
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.4 mT - - - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Exposure to the magnetic field increased cell proliferation, enhanced levels of ceramides and sphingosin-1-phosphate and activated ERK 1/2 significantly compared to the sham exposure.
The increase in cell proliferation and ERK1/2 activation was inhibited by the sphingosine kinase inhibitor SKI II and the inhibitor of ERK 1 and 2, U0126.
The authors conclude that sphingosine‐1‐phosphate could mediate 50‐Hz magnetic fieldinduced cell proliferation of human amniotic cells via triggering the ERK1/2 signal pathway.

Study character:

Study funded by

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