Exposure duration: 60 min
|Chamber||two exposure chambers which were put in a CO2 incubator|
|Setup||one chamber was used for exposure and the other for sham exposure; each chamber consisted of a set of square Helmholtz coils (20 × 20 cm2), which was encased by mu‐metal for shielding the cells placed in the coils from stray magnetic fields; a fan on the metal wall ensured the air and the temperature was uniform between inside the chamber and incubator; during exposure, cell dishes were put in the center of the chambers; the temperature in the chambers was kept at 37.0 ± 0.2°C throughout the whole exposure period; the difference of the temperature between exposure and sham exposure conditions during the entire exposure period did not exceed 0.1°C|
|Sham exposure||A sham exposure was conducted.|
|magnetic flux density||0.4 mT||-||-||-||-|
Exposure to the magnetic field increased cell proliferation, enhanced levels of ceramides and sphingosin-1-phosphate and activated ERK 1/2 significantly compared to the sham exposure.
The increase in cell proliferation and ERK1/2 activation was inhibited by the sphingosine kinase inhibitor SKI II and the inhibitor of ERK 1 and 2, U0126.
The authors conclude that sphingosine‐1‐phosphate could mediate 50‐Hz magnetic field‐induced cell proliferation of human amniotic cells via triggering the ERK1/2 signal pathway.