Nymphs of orange-spotted cockroach (Blaptica dubia) were divided into the following groups at the age of one month (n=10, respectively): 1) exposure to a static magnetic field, 2) exposure to a 50 Hz magnetic field, 3) control group.
|Chamber||3-5 nymphs in Petri dishes|
a double-U shaped permanent magnet; Petri dishes were placed between the poles (distance 4.5 cm); the magnetic field lines were parallel to the vertical component of the
geomagnetic field; the temperature was stable during the experiment with 26 ± 0.2°C
|Sham exposure||A sham exposure was conducted.|
|magnetic flux density||110 mT||-||measured||-||field homogeneity ± 26.87%|
|Chamber||see field 1|
electromagnet consisting of a regular laminated transformer core surrounded by three pairs of coils; each coil was made of 84 copper wires (1 mm in diameter); the surface of the poles was 44.6 cm2 with a 7.5 cm space
between them; the magnetic field lines were parallel to the vertical component of the geomagnetic field; the average temperature was 26.3 ± 0.05°C
The average weight of the gut was significantly reduced in exposed nymphs (groups 1 and 2) compared to the control group. The enzyme activities of superoxide dismutase and catalase were significantly increased in exposed nymphs of groups 1 and 2 compared to the control group, while the enzyme activities of glutathione reductase and glutathione S-transferase were significantly reduced. In addition, the enzyme activities of superoxide dismutase and glutathione reductase in group 2 (exposure to 50 Hz magnetic field) were significantly increased compared to group 1 (exposure to static magnetic field). The total glutathione content showed no significant differences between the groups.
The authors conclude that chronic exposure of orange-spotted cockroach nymphs to a static magnetic field or 50 Hz magnetic field could alter the weight of the gut and the investigated antioxidative markers.