Eggs from Caenorhabditis elegans of the parental generation were collected and divided into an exposure group and a control group. The hatched individuals were recorded as the first filial generation (F1) and when they laid eggs, these were collected and placed in the same environment as the F1 generation. After hatching, these individuals were defined as the second filial generation (F2). This process was continued until the F15 generation.
Test were conducted with worms form different generations. Each test was repeated three times.
Exposure duration: continuously for up to the 15th generation
|Exposure duration||continuously for up to the 15th generation|
|Setup||two coaxial Helmholtz coils generated a uniform magnetic field in the central experimental area; the coil was wrapped around a condensation water pipe to take away the heat generated during operation; the whole device was placed in an incubator and the temperature was monitored and controlled at 20±0.1°C|
|magnetic flux density||3 mT||-||-||-||-|
Body length of exposed F15 worms increased significantly compared to the control group. ATP content and ATPase activity were significantly increased in exposed F15 worms compared to the control group. The gene expression levels of the r53.4, hpo-18, atp-5, unc-32 and atp-3 genes were significantly upregulated in exposed F15 worms compared to the control group. In addition, SOD activity increased significantly and the gene expression levels of the sod-1, sod-2, and sod-3 genes were also significantly upregulated in exposed F15 worms compared to the control group.
The authors conclude that chronic exposure to a 50 Hz magnetic field over multiple generations might increase growth, metabolism and antioxidant capacity in nematodes.