Study type: Medical/biological study (experimental study)

Modification of production of reactive oxygen species in mouse peritoneal neutrophils on exposure to low-intensity modulated millimeter wave radiation. med./bio.

Published in: Bioelectrochem Bioenerg 1998; 46 (2): 267-272

Aim of study (acc. to author)

To investigate the effects of electromagnetic irradiation on the production of reactive oxygen species (ROS) in mouse neutrophils in vitro.

Background/further details

The cells were treated with a calcium ionophore (A23187) and a phorbol ester (phorbol-12-myristate-13-acetate). Both substances lead synergistically to an increased production of reactive oxygen species in non-irradiated cells.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 41.8–42.05 GHz
Modulation type: FM
Exposure duration: continuous for 20 min

General information

cells were exposed to: i) only EMF ii) EMF + calcium ionophore A23187

Exposure 1

Main characteristics
Frequency 41.8–42.05 GHz
Type
Charakteristic
Exposure duration continuous for 20 min
Modulation
Modulation type FM
Modulation frequency 100 mHz–50 Hz
Exposure setup
Exposure source
Distance between exposed object and exposure source 0.4 m
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
power density 150 µW/cm² maximum calculated - 20 - 150 µW/cm²

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The electromagnetic irradiation affected the production of reactive oxygen species (ROS) as determined by luminol-chemiluminescence. Chemiluminescence is proportional to the oxygen species production. The observed effects depend on carrier frequency and modulation frequency.

Study character:

Study funded by

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