The cells were treated with a calcium ionophore (A23187) and a phorbol ester (phorbol-12-myristate-13-acetate). Both substances lead synergistically to an increased production of reactive oxygen species in non-irradiated cells.
|Modulation frequency||100 mHz–50 Hz|
|power density||150 µW/cm²||maximum||calculated||-||20 - 150 µW/cm²|
The electromagnetic irradiation affected the production of reactive oxygen species (ROS) as determined by luminol-chemiluminescence. Chemiluminescence is proportional to the oxygen species production. The observed effects depend on carrier frequency and modulation frequency.