Study type: Medical/biological study (experimental study)

Changes of selected biochemical parameters of the honeybee under the influence of an electric field at 50 Hz and variable intensities. med./bio.

Published in: Apidologie 2020; 51: 956–967

Aim of study (acc. to author)

The effects of exposure of honeybees to a 50 Hz electric field on the antioxidative and proteolytic system should be investigated.

Background/further details

One-day-old honeybee workers were divided into the following groups: exposure to an electric field of 1) 5.0 kV/m for 1 hour, 2) 5.0 kV/m for 3 hours, 3) 5.0 kV/m for 6 hours, 4) 11.5 kV/m for 1 hour, 5) 11.5 kV/m for 3 hours, 6) 11.5 kV/m for 6 hours, 7) 23.0 kV/m for 1 hour, 8) 23.0 kV/m for 3 hours, 9) 23.0 kV/m for 6 hours, 10) 34.5 kV/m for 1 hour, 11) 34.5 kV/m for 3 hours and 12) 34.5 kV/m for 6 hours. For each exposure duration (1 hour, 3 hours, 6 hours), a separate control group was used. Each group consisted of 10 cages with 100 bees, respectively.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 1 hour
Exposure 2: 50 Hz
Exposure duration: 3 hours
Exposure 3: 50 Hz
Exposure duration: 6 hours
Exposure 4: 50 Hz
Exposure duration: 1 hour
Exposure 5: 50 Hz
Exposure duration: 3 hours
Exposure 6: 50 Hz
Exposure duration: 6 hours
Exposure 7: 50 Hz
Exposure duration: 1 hour
Exposure 8: 50 Hz
Exposure duration: 3 hours
Exposure 9: 50 Hz
Exposure duration: 6 hours
Exposure 10: 50 Hz
Exposure duration: 1 hour
Exposure 11: 50 Hz
Exposure duration: 3 hours
Exposure 12: 50 Hz
Exposure duration: 6 hours

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration 1 hour
Exposure setup
Exposure source
  • plate capacitor
Chamber wooden cages (20 × 15 × 7 cm)
Setup a homogeneous (± 5%) electric field was generated by a plate capacitor with a distance of 20 cm between two electrodes constructed as a squared cage made out of wire mesh (dimension 100 cm × 100 cm) with a 10 mm× 10 mm grid diameter
Additional info the control groups were exposed to an electric field of < 2.00 kV/m
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 5 kV/m - measured - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Exposure duration 3 hours
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 5 kV/m - measured - -

Exposure 3

Main characteristics
Frequency 50 Hz
Type
Exposure duration 6 hours
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 5 kV/m - measured - -

Exposure 4

Main characteristics
Frequency 50 Hz
Type
Exposure duration 1 hour
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 11.5 kV/m - measured - -

Exposure 5

Main characteristics
Frequency 50 Hz
Type
Exposure duration 3 hours
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 11.5 kV/m - measured - -

Exposure 6

Main characteristics
Frequency 50 Hz
Type
Exposure duration 6 hours
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 11.5 kV/m - measured - -

Exposure 7

Main characteristics
Frequency 50 Hz
Type
Exposure duration 1 hour
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 23 kV/m - measured - -

Exposure 8

Main characteristics
Frequency 50 Hz
Type
Exposure duration 3 hours
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 23 kV/m - measured - -

Exposure 9

Main characteristics
Frequency 50 Hz
Type
Exposure duration 6 hours
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 23 kV/m - measured - -

Exposure 10

Main characteristics
Frequency 50 Hz
Type
Exposure duration 1 hour
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 34.5 kV/m - measured - -

Exposure 11

Main characteristics
Frequency 50 Hz
Type
Exposure duration 3 hours
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 34.5 kV/m - measured - -

Exposure 12

Main characteristics
Frequency 50 Hz
Type
Exposure duration 6 hours
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 34.5 kV/m - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

All exposure groups showed a significant increase compared to the respective control groups in one or more enzyme activities of the antioxidant and proteolytic systems.
The authors conclude that exposure to a 50 Hz electric field might affect the antioxidative and proteolytic system of honeybees.

Study character:

Study funded by

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