Study type: Medical/biological study (experimental study)

Synergic effects of extremely low-frequency electromagnetic field and betaine on in vitro osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells. med./bio.

Published in: In Vitro Cell Dev Biol Anim 2021 [in press]

Aim of study (acc. to author)

The effects of (co-)exposure to a 50 Hz magnetic field and betaine on the osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells should be investigated.

Background/further details

Cells were divided into the following groups: 1) exposure to the magnetic field, 2) exposure to betaine (10 mM), 3) co-exposure to the magnetic field and betaine, 4) control group (only treatment with osteogenic differentiation medium), 5) negative control (no treatment). Groups 1-4 were treated with osteogenic differentiation medium. Betaine could be a promising therapeutic agent for osteoporosis as it stimulates different signaling pathways leading to enhanced bone formation.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 8 hours/day for up to 21 days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 8 hours/day for up to 21 days
Exposure setup
Exposure source
Setup a uniform magnetic field was produced by a coil with dimensions of 68 x 100 mm2, corresponding to the dimensions of the flask used for exposure of cells; the coil was made of 2200 turns of 0.4 mm diameter enameled wire wound around a plastic spool; a magnetic silicon alloy core was placed into the spool; to prevent heat from the eddy current, the magnetic core was made of a large number of very thin EI-shaped sheets; the whole coil was sealed with an air-during varnish; a 24-well plate cover with dimensions of 85 × 128 mm2 was placed on the coil as the cell flask holder
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - measured - -

Reference articles

  • Luo F et al. (2012): Effects of pulsed electromagnetic field frequencies on the osteogenic differentiation of human mesenchymal stem cells.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

A significant increase in calcium deposition was observed in the co-exposure group (group 3) compared to the other groups. The enzyme activity of alkaline phosphatase was significantly decreased in the magnetic field (group 1) and betaine (group 2) groups compared to the control group and but it was significantly higher in group 3 compared to group 1. The gene expression of RUNX2 and OCN was significantly reduced in group 1 compared to the control group but group 3 showed a significant increase in RUNX2 gene expression compared to group 1.
The authors concluded that co-exposure to a 50 Hz magnetic field and betaine might have synergistic effects on the osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.

Study character:

Study funded by

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