Home
Medical/biological study (experimental study)

Effects of extremely low-frequency magnetic field on growth and differentiation of human mesenchymal stem cells.

Published in: Electromagn Biol Med 2010; 29 (4): 165-176

Aim of study (acc. to author)

To study the effects of extremely low frequency magnetic field exposure on growth, metabolism and cell differentiation of human mesenchymal stem cells.
Background/further details: For cell differentiation experiments, the stem cells were cultivated in osteogenic medium.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 12 h/day on 23 days
Exposure 1
Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 12 h/day on 23 days
Exposure setup
Exposure source
Setup 40 cm long coils with a diameter of 21 cm, consisting of 880 turns/m of 1.1 mm enamel insulated copper wire wound on a cylindrical epoxy resin support; coils arranged horizontally to generate a vertical field inside a 5 % CO2 incubator at 37° C; samples placed on a Plexiglas slab in the center of the coil system
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 20 mT - measured - -
Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated material:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

The data showed that exposure to extremely low frequency magnetic fields could influence the growth (decreased cell number under exposure) and metabolism (increased osmolality and increased extracellular sodium and potassium concentrations) of human mesenchymal stem cells, but had no significant effect on cell differentiation.
Study character:

Study funded by

  • not stated/no funding

Related articles

  • Moraveji M et al. (2016): Effect of extremely low frequency electromagnetic field on MAP2 and Nestin gene expression of hair follicle dermal papilla cells.
  • Mascotte-Cruz JU et al. (2016): Combined effects of flow-induced shear stress and electromagnetic field on neural differentiation of mesenchymal stem cells.
  • An GZ et al. (2015): Effects of long-term 50 Hz power-line frequency electromagnetic field on cell behavior in Balb/c 3T3 cells.
  • Razavi S et al. (2014): Extremely low-frequency electromagnetic field influences the survival and proliferation effect of human adipose derived stem cells.
  • Bishi DK et al. (2014): Low frequency magnetic force augments hepatic differentiation of mesenchymal stem cells on a biomagnetic nanofibrous scaffold.
  • Bai WF et al. (2013): Fifty-Hertz electromagnetic fields facilitate the induction of rat bone mesenchymal stromal cells to differentiate into functional neurons.
  • Kim HJ et al. (2013): Extremely low-frequency electromagnetic fields induce neural differentiation in bone marrow derived mesenchymal stem cells.
  • Liu C et al. (2013): Effect of 1 mT Sinusoidal Electromagnetic Fields on Proliferation and Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stromal Cells.
  • Zhang M et al. (2013): Effects of low frequency electromagnetic field on proliferation of human epidermal stem cells: An in vitro study.
  • Bai WF et al. (2012): Effects of 50 Hz electromagnetic fields on human epidermal stem cells cultured on collagen sponge scaffolds.
  • Zhong C et al. (2012): Effects of Low-Intensity Electromagnetic Fields on the Proliferation and Differentiation of Cultured Mouse Bone Marrow Stromal Stem Cells.
  • Cho H et al. (2012): Neural stimulation on human bone marrow-derived mesenchymal stem cells by extremely low frequency electromagnetic fields.
  • Yang Y et al. (2010): EMF acts on rat bone marrow mesenchymal stem cells to promote differentiation to osteoblasts and to inhibit differentiation to adipocytes.