Study type: Medical/biological study (experimental study)

Effects of extremely low-frequency magnetic field on growth and differentiation of human mesenchymal stem cells. med./bio.

Published in: Electromagn Biol Med 2010; 29 (4): 165-176

Aim of study (acc. to author)

To study the effects of extremely low frequency magnetic field exposure on growth, metabolism and cell differentiation of human mesenchymal stem cells.

Background/further details

For cell differentiation experiments, the stem cells were cultivated in osteogenic medium.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 12 h/day on 23 days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 12 h/day on 23 days
Exposure setup
Exposure source
Setup 40 cm long coils with a diameter of 21 cm, consisting of 880 turns/m of 1.1 mm enamel insulated copper wire wound on a cylindrical epoxy resin support; coils arranged horizontally to generate a vertical field inside a 5 % CO2 incubator at 37° C; samples placed on a Plexiglas slab in the center of the coil system
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 20 mT - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated material:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

The data showed that exposure to extremely low frequency magnetic fields could influence the growth (decreased cell number under exposure) and metabolism (increased osmolality and increased extracellular sodium and potassium concentrations) of human mesenchymal stem cells, but had no significant effect on cell differentiation.

Study character:

Study funded by

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