Study type: Medical/biological study (experimental study)

Enhanced growth and osteogenic differentiation of Induced Pluripotent Stem cells by Extremely Low-Frequency Electromagnetic Field. med./bio.

Published in: Cell Mol Biol (Noisy-le-grand) 2015; 61 (1): 36-41

Aim of study (acc. to author)

The effects of exposure of induced pluripotent stem cells to a pulsed 50 Hz magnetic field on osteogenic differentiation should be investigated.

Background/further details

Cells were divided into 4 groups: 1) exposure to the magnetic field, 2) treatment with osteogenic medium (3 mM beta-glycerophosphate, 50 µg/ml ascorbic acid and 10-9 M dexamethasone), 3) co-exposure to the magnetic field and osteogenic medium, 4) control group.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Modulation type: pulsed
Exposure duration: for 8 hours/day for 1, 3, 5, 7 or 14 days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration for 8 hours/day for 1, 3, 5, 7 or 14 days
Modulation
Modulation type pulsed
Pulse width 350 ns
Exposure setup
Exposure source
  • ELF generator
Setup 6 well culture plates were placed in exposure apparatus inside a CO2 incubator (5% CO2, 37°C)
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1.5 mT - - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

After 5 and 7 days, all exposure groups (groups 1-3) showed a significantly increased proliferation compared to the control group. However, the co-exposure group (group 3) showed the highest value, respectively, which was even significantly higher than in groups 1 and 2.
Mineralization of the extracellular matrix was most distinct in group 3 as well and the calcium content, enzyme activity of alkaline phosphatase and the gene expression of most osteogenesis-related genes (collagen type 1, osteocalcin, alkaline phosphatase, osteoprotegerin, matrix metalloproteinases 1 and 3) were also most distinct in group 3 and significantly increased after 7 and/or 14 days compared to groups 1 and 2.
The authors conclude that exposure to a pulsed 50 Hz magnetic field in combination with osteogenic medium might be beneficial for osteogenic differentiation of induced pluripotent stem cells. These findings may be of therapeutical use.

Study character:

Study funded by

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