A maximum temperature increase of 0,5°C was observed at SARs of 100 mW/g. Intact cells will exclude trypan blue, thus an uptake of this dye reveals altered membrane properties. The release of intracellular enzymes, such as LDH or creatine phosphokinase, serve as an indicator of abnormal membrane permeability as well.
|Exposure duration||90 min|
|Setup||The sample tube (1.05/1.36 cm inner/outer diameter) with magnetic stirrers was placed against the dielectric plate in the waveguide filled with distilled water and maintained at 37°C.|
|Additional info||the control sample tube was placed 9.5 cm away from the dielectric plate and the temperature was the same as in the case of the exposed samples|
It was found that even low SARs of 10 mW/g increased the uptake of trypan blue and at SARs of 100 mW/g a release of LDH was observed. Electron microscopy confirmed cellular damage only at SAR values of 100 mW/g.