Study type: Medical/biological study (experimental study)

Effects of low-frequency magnetic fields on implantation in rats med./bio.

Published in: Reprod Toxicol 2001; 15 (1): 49-59

Aim of study (acc. to author)

To study the hypothesis that magnetic fields enhance the implantation of embryos which would normally rejected (i.e. embryos which are not sufficiently matured to implant after reaching the uterus). For this purpose female rats were exposed to magnetic fileds during the early pregnancy period and pre-implantated and implanted embryos were collected from oviducts and uteri, respectively.

Background/further details

Female rats were continuously exposed 24 h/day in the early period of pregnancy (i.e. up to 176 h). Dams were killed and examined both during light and dark periods (70, 80, 94, 104, 118, 128, 142, 152, 166, and 176 h after ovulation).



Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 24 h/day from day 0 of pregnancy until at least 70 h up to 176 h after ovulation

Exposure 1

Main characteristics
Frequency 50 Hz
Exposure duration 24 h/day from day 0 of pregnancy until at least 70 h up to 176 h after ovulation
Exposure setup
Exposure source
Setup two pairs of Helmholtz coils in wooden frames; coils with a cross section of 0.425 x 1.205 m, 0.155 m apart; horizontal distance between coil pairs 0.485 m; two cages inside each coil; for 13 µT coils with 1 turn; for 130 µT coils with 10 turns
Sham exposure A sham exposure was conducted.
Measurand Value Type Method Mass Remarks
magnetic flux density 13 µT effective value - - -
magnetic flux density 130 µT effective value - - -
magnetic field strength 100 A/m - - - -
magnetic field strength 10 A/m - - - -
magnetic flux density 48 µT minimum measured - e: geomagnetic flux density with EMF exposure: 48 - 55 µTd: geomagnetische Flußdichte bei EMF Exposition: 48 - 55 µT
magnetic flux density 45 µT minimum measured - e: geomagnetic flux density without EMF exposure: 45 - 50 µTd: geomagnetische Flußdichte ohne EMF Exposition: 45 - 50 µT

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Magnetic field exposure did not alter the mean total number of implantations. Nocturnal melatonin levels were decreased by 38 and 34% at 100 and 10 A/m, respectively. Serum levels of progesterone and estradiol were not significantly changed. Whilst early implantation period, the cytosolic ratio of estrogen- to progesterone receptors was found to be decreased at 100 A/m. Whereas the nuclear ratio of estrogen-/progesterone receptors was decreased in both exposure groups. 15 and 19% of the embryos at 100 and 10 A/m were yet morulae and not implanted.
The authors summarize, that magnetic field exposure do not impair implantation in rats although there may be some borderline changes in embryonic development and endocrine parameters.

Study character:

Study funded by

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