Study type: Medical/biological study (experimental study)

Poly ADP ribosylation as a possible mechanism of microwave--biointeraction. med./bio.

Published in: Indian J Physiol Pharmacol 1994; 38 (3): 181-184

Aim of study (acc. to author)

To observe a possible effect of microwave irradiation on the poly ADPR polymerase activity.

Background/further details

Poly ADP-ribosylation was studied, which is a post translational modification of chromatin protein catalysed by the enzyme poly ADPR polymerase using NAD+ as the substrate. Poly ADP-ribosylation has been shown to be involved in several aspects of chromatin function and structure.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 2.45 GHz
Modulation type: CW
Exposure duration: continuous, 6 days

Exposure 1

Main characteristics
Frequency 2.45 GHz
Type
Waveform
Exposure duration continuous, 6 days
Modulation
Modulation type CW
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
power density 10 W/m² unspecified - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The enzyme activity was increased in liver and testis and was decreased in diencephalon and brain cortex. No altered enzyme activity was found in kidney and spleen.
The results of the NAD+ level estimation was diametrically opposed to the enzyme activity: A drop of NAD+ levels was found in liver and testis whereas cortex and diencephalon showed an increase. The determination of protein by SDS-PAGE showed no changes at all to the controls.

Study character:

Study funded by