Study type: Medical/biological study (experimental study)

Dose dependence of acetylcholinesterase activity in neuroblastoma cells exposed to modulated radio-frequency electromagnetic radiation. med./bio.

Published in: Bioelectromagnetics 1992; 13 (4): 317-322

Aim of study (acc. to author)

To test whether a cholinergic neurotransmitter system would be responsive to the exposure conditions that induce calcium-ion flux and to determine whether the response would be windowed or would follow the more classical dose-response kinetics.

Background/further details

Acetylcholinesterase (AChE) as biochemical marker was used because of its presence in the cell membrane and its physiologically important role in the acetylcholinergic neurotransmitter system of intercellular communication.



Exposure Parameters
Exposure 1: 147 MHz
Modulation type: AM
Exposure duration: continuous for 30 min
  • SAR: 0.1 W/kg (0.001, 0.005, 0.01, 0.02, 0.05, or 0.10 W/kg)

Exposure 1

Main characteristics
Frequency 147 MHz
Exposure duration continuous for 30 min
Modulation type AM
Modulation frequency 16 Hz
Modulation depth 80 %
Additional info


Exposure setup
Exposure source
Chamber A transmission-line exposure system was housed in a large incubator maintained at 37°C, as described by Dutta et al. [1984].
Setup Two tissue culture flasks of 25 cm² growth surface containing cells in 5 ml of growth medium were exposed per trial.
Additional info Two control flasks were placed in the incubator outside the transmission line.
Measurand Value Type Method Mass Remarks
SAR 0.1 W/kg - - - 0.001, 0.005, 0.01, 0.02, 0.05, or 0.10 W/kg

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The results indicate that exposure to modulated radiofrequency fields at different SARs affects AChE activity of neuroblastoma cells. Enhanced activity was observed within a time window between 7.0 and 7.5 h after the cells were plated and only when the radiation occured at power densities identified in a previous report (publication 232) as being effective for altering the release of calcium ions. Radiofrequency was not effective in changing AChE activity at earlier time points, e.g. 1 and 2 h after plating (data not shown). Thus radiofrequency radiation under these conditions affects both calcium-ion release and AChE activity in a common dose-dependent manner.

Study character:

Study funded by

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