Study type: Medical/biological study (experimental study)

Differentiation of chromaffin cells elicited by ELF MF modifies gene expression pattern. med./bio.

Published in: Cell Biol Int 2004; 28 (4): 273-279

Aim of study (acc. to author)

This study was performed to identify genes that could be involved in extremely low frequency magnetic field induced chromaffin cell differentiation.

Background/further details

The differentiation of chromaffin cells exposed to extremely low frequency magnetic field into sympathetic neuron-like cells was demonstrated by a prior study (publication 3576) and should be further investigated on molecular genetic level.
The chromaffin cells used were maintained from neonatal Wistar rats and investigated in three groups: 1.) sham exposed, 2.) nerve growth factor (NGF) treated and 3.) electromagnetic field exposed.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: repeated daily exposure, 4 h/day for 7 days

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration repeated daily exposure, 4 h/day for 7 days
Exposure setup
Exposure source
Chamber Incubator
Setup Petri dishes were placed between the coils at a distance of 21 cm.
Additional info The control and NGF group were placed in a separate incubator.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 700 µT unspecified measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Prevalent down-regulated gene transcription was observed in the exposed and NGF treated cells compared to the control group (53 transcripts with differential expression). The exposure to extremely low frequency magnetic field showed a specific transcription response (among others, 8 up-regulated transcripts), qualitatively different from that elicited by NGF treated cells.
The sequencing of the electromagnetic field differential expressed genes revealed an increased density of cytosine-thymidine-cytosine-thymidine (CTCT) elements in promoters. These elements are thought to be required for electromagnetic field-dependent gene expression.

Study character:

Study funded by

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