Study type: Medical/biological study (experimental study)

In vitro study of the effects of ELF electric fields on gene expression in human epidermal cells. med./bio.

Published in: Bioelectromagnetics 2011: 28-36

Aim of study (acc. to author)

To study the effects of extremely low frequency electric fields on gene expression in human epidermal cells.

Background/further details

Human epidermal explants were harvested from human abdominoplasty of three patients after plastic surgery. Gene expression tests were performed on days 4, 7 and 12.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 40 Hz
Modulation type: pulsed
Exposure duration: (4 s on - 4 s off) for 40 min/day on 11 days

Exposure 1

Main characteristics
Frequency 40 Hz
Type
Exposure duration (4 s on - 4 s off) for 40 min/day on 11 days
Modulation
Modulation type pulsed
Pulse width 250 ms
Pulse type biphasic
Additional info

modulating frequency 0.125 Hz

Exposure setup
Exposure source
Setup six 3 mm circles of epidermis layer placed on a dermal support; electrodes in contact with the support
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 275 V/m - measured - in the explants
electric field strength 400 V/m maximum measured - around the electrodes
cf. remarks - - - - peak current: 20 mA

Reference articles

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

After the application of extremely low frequency electric fields, the microarray analysis detected a modification of the gene expression of the epidermis cells. Particularly, four up-regulated genes (DKK1, TXNRD1, ATF3, and MME) and one down-regulated gene (MACF1) were identified which are involved in the regulation of proliferation and differentiation. Expression of these five genes was confirmed by RT-PCR. .

Study character:

Study funded by

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