Study type: Medical/biological study (experimental study)

The repair of gamma-ray-induced chromosomal damage in human lymphocytes after exposure to extremely low frequency electromagnetic fields. med./bio.

Published in: Cytogenet Genome Res 2004; 104 (1-4): 188-192

Aim of study (acc. to author)

To study the possibility that a electric or magnetic field in some way reduces the fidelity of repair of DNA damage (q.v. DNA repair) caused by a established mutagen.

Background/further details

The in vitro assay of chromosomal aberrations induced in human lymphocytes by gamma irradiation was used.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 12 h
Exposure 2: 50 Hz
Exposure duration: continuous for 12 h
Exposure 3: 50 Hz
Exposure duration: continuous for 12 h

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 12 h
Additional info Cells were irradiated with 2.0 Gy gamma rays.
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Additional info During each run each coil was loaded with six replicate cultures previously irradiated with gamma rays and two replicates with unirradiated cells. In addition four replicate cultures of gamma irradiated cells were setup under identical conditions but retained at the NRPB laboratory and incubated in parallel.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 230 µT unspecified unspecified - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 12 h
Additional info Cells were irradiated with 2.0 Gy gamma rays.
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 470 µT unspecified unspecified - -

Exposure 3

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 12 h
Additional info Cells were irradiated with 2.0 Gy gamma rays.
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 700 µT unspecified unspecified - -

Reference articles

  • Wolff H et al. (1999): The design, construction and calibration of a carefully controlled source for exposure of mammalian cells to extremely low-frequency electromagnetic fields.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No differences were revealed in the frequencies of gamma-induced chromosome aberrations observed in cells held in the electromagnetic fields compared with replicates held in a sham coil. Thus, the presence of these fields does not compromise directly or indirectly the repair of the gamma-ray induced lesions.

Study character:

Study funded by

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