Medical/biological study (experimental study)

Possible associations between ELF electromagnetic fields, DNA damage response processes and childhood leukaemia med./bio.

By: Hone P, Edwards A, Halls J, Cox R, Lloyd D

Published in: Br J Cancer 2003; 88 (12): 1939-1941

Exposure

Exposure	Parameters
Exposure 1: 50 Hz Exposure duration: continuous for 12 h	magnetic flux density: 0.23 mT unspecified magnetic flux density: 0.47 mT unspecified magnetic flux density: 0.7 mT unspecified

Exposure 1

Main characteristics

Frequency	50 Hz
Type	magnetic field
Waveform	unspecified
Exposure duration	continuous for 12 h

Exposure setup

Exposure source	solenoid
Setup	Cultures were placed in the ELF rig where the magnetic field varied within 1%. Six cultures were irradiated to gamma rays of 2 Gy and two were kept unirradiated.
Sham exposure	A sham exposure was conducted.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	0.23 mT	unspecified	unspecified	-	-
magnetic flux density	0.47 mT	unspecified	unspecified	-	-
magnetic flux density	0.7 mT	unspecified	unspecified	-	-

Reference articles

Wolff H et al. (1999): The design, construction and calibration of a carefully controlled source for exposure of mammalian cells to extremely low-frequency electromagnetic fields

Exposed system:

blood sample

Methods Endpoint/measurement parameters/methodology

genotoxicity/mutation: DNA damage (chromosome aberration, dicentric chromosome exchange score)

Investigated system:

DNA/RNA
intact cell/cell culture

Time of investigation:

after exposure

Main outcome of study (acc. to author)

The data from 12 hours post gamma radiation exposure to electromagnetic fields of different magnetic flux densities provide no support of the hypothesis that electromagnetic field may have influence on response to gamma radiation induced DNA damage in human lymphocytes.

Study character:

medical/biological study
experimental study
full/main study
short communication
blind study

Study funded by

EMF Biological Research Trust (EMFBRT), UK

Celikler S et al. (2009): A biomonitoring study of genotoxic risk to workers of transformers and distribution line stations
Delimaris J et al. (2006): Effects of pulsed electric fields on DNA of human lymphocytes
Villarini M et al. (2006): Effects of co-exposure to extremely low frequency (50 Hz) magnetic fields and xenobiotics determined in vitro by the alkaline comet assay
Hone P et al. (2006): Chromatid damage in human lymphocytes is not affected by 50 Hz electromagnetic fields
Moretti M et al. (2005): Effects of co-exposure to extremely low frequency (ELF) magnetic fields and benzene or benzene metabolites determined in vitro by the alkaline comet assay
Koyama S et al. (2005): Combined exposure of ELF magnetic fields and x-rays increased mutant yields compared with x-rays alone in pTN89 plasmids
Luceri C et al. (2005): Extremely low-frequency electromagnetic fields do not affect DNA damage and gene expression profiles of yeast and human lymphocytes
Stronati L et al. (2004): Absence of genotoxicity in human blood cells exposed to 50 Hz magnetic fields as assessed by comet assay, chromosome aberration, micronucleus, and sister chromatid exchange analyses
Lloyd D et al. (2004): The repair of gamma-ray-induced chromosomal damage in human lymphocytes after exposure to extremely low frequency electromagnetic fields
Testa A et al. (2004): Evaluation of genotoxic effect of low level 50 Hz magnetic fields on human blood cells using different cytogenetic assays
Maes A et al. (2000): Cytogenetic effects of 50 Hz magnetic fields of different magnetic flux densities