No effect of pulsed electromagnetic fields on PC12 and HL-60 cells.
Published in: Radiat Environ Biophys 2006; 45 (1): 63-71
Aim of study (acc. to author)
magnetic field of a single pulse increased rapidly during the first 100 µs to a maximum and exponentially decreased afterwards within 1 ms. The time between two pulses was about 5 s. The frequency of a single pulse was estimated to be 12.4 kHz.
coil each with 50 mm diameter with a hole diameter of 10 mm
coil was fixed with the front of the coil in upside position. 35 mm Petri dishes were placed on top of the double coil and shielded against heat and vibrations with a 2 mm thick plate of plastic foam. Sham exposed dishes were placed in the same room but outside the influence of the coil.
sham exposure was conducted.
The dishes were
exposed to non- homogeneous MF. The MF and EF varied from +85% to -85% and 15 to 100%, respectively if the dishes were positioned in the middle position between the two turns and from 50 to 100% and 0 to 50%, respectively if positioned in the middle of each turn. The dishes were exposed in the middle of the coil, on the left side on the coil with the north magnetic direction, or on the right side of the coil with south magnetic direction.
Main outcome of study (acc. to author)
Study funded by
Kaszuba-Zwoinska J et al.
Pulsating electromagnetic field stimulation prevents cell death of puromycin treated U937 cell line.
Sun LY et al.
Effect of pulsed electromagnetic field on the proliferation and differentiation potential of human bone marrow mesenchymal stem cells.
Selvamurugan N et al.
Effects of BMP-2 and pulsed electromagnetic field (PEMF) on rat primary osteoblastic cell proliferation and gene expression.
De Mattei M et al.
Changes in polyamines, c-myc and c-fos gene expression in osteoblast-like cells exposed to pulsed electromagnetic fields.
Tepper OM et al.
Electromagnetic fields increase in vitro and in vivo angiogenesis through endothelial release of FGF-2.
Response of cyclic AMP by DMSO differentiated HL-60 cells exposed to electric interferential current after prestimulation.
Lohmann CH et al.
Pulsed electromagnetic fields affect phenotype and connexin 43 protein expression in MLO-Y4 osteocyte-like cells and ROS 17/2.8 osteoblast-like cells.
Glück B et al.
Inhibition of proliferation of human lymphoma cells U937 by a 50 Hz electromagnetic field.
Miyagi N et al.
Effects of PEMF on a murine osteosarcoma cell line: drug-resistant (P-glycoprotein-positive) and non-resistant cells.
Modulation of cytokine production by interferential current in differentiated HL-60 cells.
Treatment of zymosan-activated HL-60 cells with low frequencies electric fields does not change cellular ATP and ADP levels and reactive oxygen species.
Sontag W et al.
Response of cytosolic calcium, cyclic AMP, and cyclic GMP in dimethylsulfoxide-differentiated HL-60 cells to modulated low frequency electric currents.
Opler M et al.
Reduced dopamine levels in PC12 cells exposed to low frequency electromagnetic fields.
Two experimental systems for exposure of cells to extremely low frequency electric fields suitable for in situ measurements of fluorescence signals.
Knedlitschek G et al.
Cyclic AMP response in cells exposed to electric fields of different frequencies and intensities.
Schimmelpfeng J et al.
The action of 50 Hz magnetic and electric fields upon cell proliferation and cyclic AMP content of cultured mammalian cells.
Dixey R et al.
³H-noradrenaline release potentiated in a clonal nerve cell line by low-intensity pulsed magnetic fields.