研究のタイプ: 医学／生物学の研究 (experimental study)

[PC12とHL-60細胞に対してパルス電磁界の影響はない] med./bio.

No effect of pulsed electromagnetic fields on PC12 and HL-60 cells

著者: Sontag W, Kalka D
掲載誌: Radiat Environ Biophys 2006; 45 (1): 63-71
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この研究は、経頭蓋磁気刺激（TMS）で使用されるものと同様のパルス電磁界（PEMF）による影響を、2種類の腫瘍細胞株（ヒト前骨髄球性白血病細胞株（HL-60）およびラット褐色細胞腫細胞株（PC12））を用いた実験で調べた。ダブルコイルの異なる位置に培養皿を置き、細胞に非一様なパルス磁界のばく露を与えた。磁束密度は計算上、最大4 T、最小0.25 Tであり、最大値を100%として、100、50、20、10、5 %でのばく露を行った。パルス形状は単相（立ち上がり時間100 μs、立ち下がり時間1ms(指数関数的)、パルス幅は5 μs）、パルス繰り返し周波数は025 Hzであり、ばく露時間は、与えたパルス数によって5秒から40秒であった。ばく露後に、2つの細胞株の細胞生存率および細胞内サイクリックAMP含有量を測定した。さらに、HL-60細胞では細胞内Hsp72含有量を、PC12細胞では神経伝達物質ドーパミン、ノルアドレナリン、アセチルコリンの放出を測定した。その結果、さまざまな条件でのPEMFばく露後の2つの腫瘍細胞株における細胞生存率またはcAMP、Hsp72、ドーパミン、ノルアドレナリン、およびアセチルコリンの含有量の変化は示されなかった、と報告している。

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研究目的（著者による）

To study the effects of pulsed electromagnetic fields (PEMF) similar to those used in transcranial magnetic stimulation (TMS) on two tumour cell lines,

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 250 mHz
Modulation type: pulsed
ばく露時間: 5 s to 40 s depending on the number of pulses applied

ばく露1

主たる特性
周波数 250 mHz
タイプ
  • magnetic field
波形
  • pulsed
ばく露時間 5 s to 40 s depending on the number of pulses applied
Modulation
Modulation type pulsed
Pulse type monophasic
Additional information

The magnetic field of a single pulse increased rapidly during the first 100 µs to a maximum and exponentially decreased afterwards within 1 ms. The time between two pulses was about 5 s. The frequency of a single pulse was estimated to be 12.4 kHz.
ばく露装置
ばく露の発生源／構造
  • double coil each with 50 mm diameter with a hole diameter of 10 mm
ばく露装置の詳細 The double coil was fixed with the front of the coil in upside position. 35 mm Petri dishes were placed on top of the double coil and shielded against heat and vibrations with a 2 mm thick plate of plastic foam. Sham exposed dishes were placed in the same room but outside the influence of the coil.
Sham exposure A sham exposure was conducted.
Additional information The dishes were exposed to non-homogeneous MF. The MF and EF varied from +85% to -85% and 15 to 100%, respectively if the dishes were positioned in the middle position between the two turns and from 50 to 100% and 0 to 50%, respectively if positioned in the middle of each turn. The dishes were exposed in the middle of the coil, on the left side on the coil with the north magnetic direction, or on the right side of the coil with south magnetic direction.
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 4 T maximum 計算値 - 100% intensity; corresponding EF of 575 V/m
磁束密度 0.25 T minimum 計算値 - -
Additional parameter details

The intensities used were 100%, 50%, 20%, 10% and 5%.

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

The data showed no effect on cell viability or on the content of cAMP, Hsp72, dopamine, noradrenaline, and acetylcholine in the two tumour cell lines after PEMF exposure under various conditions. Thus, single pulse PEMF exposure at the conditions employed here is not toxic for the two different cell culture systems (HL-60 and PC12 cells). This is in agreement with the conclusion that single-pulse and repetitive TMS in animals and humans is generally regarded as safe without lasting side effects.

研究の種別:

研究助成

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