Study type: Medical/biological study (experimental study)

Changes in polyamines, c-myc and c-fos gene expression in osteoblast-like cells exposed to pulsed electromagnetic fields. med./bio.

Published in: Bioelectromagnetics 2005; 26 (3): 207-214

Aim of study (acc. to author)

To study the effects of pulsed electromagnetic fields (PEMF) on cell proliferation, polyamine trend, and c-myc and c-fos gene expression of human osteoblast-like cell cultures.

Background/further details

Pulsed electromagnetic field stimulation promotes the healing of fractures in humans. The processes of tissue repair include protein synthesis and cell differentiation. The polyamines are compounds playing a relevant role in both protein synthesis processes and cell differentiation through c-myc and c-fos gene activation.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 75 Hz
Modulation type: pulsed
Exposure duration: continuous for 0.5 h, 1 h, 3 h, 6 h, 12 h and 24 h

Exposure 1

Main characteristics
Frequency 75 Hz
Type
Waveform
Exposure duration continuous for 0.5 h, 1 h, 3 h, 6 h, 12 h and 24 h
Modulation
Modulation type pulsed
Pulse width 1.3 ms
Duty cycle 10 %
Exposure setup
Exposure source
Chamber 5% CO2 Incubator maintained at 37°C
Setup plates with cells placed between the coils
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 2 mV/m peak value calculated - -
magnetic flux density 2.3 mT peak value measured - -

Reference articles

  • De Mattei M et al. (1999): Correlation between pulsed electromagnetic fields exposure time and cell proliferation increase in human osteosarcoma cell lines and human normal osteoblast cells in vitro.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The pulsed electromagnetic field (PEMF) increased [³H]-thymidine incorporation, while putrescine decreased after treatment; spermine and spermidine were not significantly affected. c-myc was activated after 1 h and downregulated thereafter, while c-fos mRNA levels increased after 0.5 h and then decreased.
Putrescine, spermidine, spermine trends, and [³H]-thymidine incorporation were significantly related to pulsed electromagnetic field (PEMF) treatment.
These observations provide evidence that in vitro pulsed electromagnetic fields affects the mechanisms involved in cell proliferation and cell differentiation.

Study character:

Study funded by

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