Pulsed electromagnetic field stimulation promotes the healing of fractures in humans. The processes of tissue repair include protein synthesis and cell differentiation. The polyamines are compounds playing a relevant role in both protein synthesis processes and cell differentiation through c-myc and c-fos gene activation.
|Pulse width||1.3 ms|
|Duty cycle||10 %|
The pulsed electromagnetic field (PEMF) increased [³H]-thymidine incorporation, while putrescine decreased after treatment; spermine and spermidine were not significantly affected. c-myc was activated after 1 h and downregulated thereafter, while c-fos mRNA levels increased after 0.5 h and then decreased.
Putrescine, spermidine, spermine trends, and [³H]-thymidine incorporation were significantly related to pulsed electromagnetic field (PEMF) treatment.
These observations provide evidence that in vitro pulsed electromagnetic fields affects the mechanisms involved in cell proliferation and cell differentiation.