Study type: Medical/biological study (experimental study)

Induced mitogenic activity in AML-12 mouse hepatocytes exposed to low-dose ultra-wideband electromagnetic radiation. med./bio.

Published in: Int J Environ Res Public Health 2005; 2 (1): 24-30

Aim of study (acc. to author)

To study the effect of ultra-wideband (UWB) irradiation on mitogenic activity in AML-12 mouse hepatocytes.

Background/further details

On the day of the experiment, medium was replaced with fresh hepatocyte growth medium (HGM) or serum-free growth medium (SFM). In some experiments, medium was supplemented with insulin-transferrin-selenium (a mitogen).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 1 kHz–100 MHz
Modulation type: pulsed
Exposure duration: continuous for 2 h

Exposure 1

Main characteristics
Frequency 1 kHz–100 MHz
Type
Waveform
Charakteristic
  • guided field
Exposure duration continuous for 2 h
Modulation
Modulation type pulsed
Pulse width 10 ns
Repetition frequency 1 kHz
Exposure setup
Exposure source
Chamber The exposure facility was shielded with copper and steel plating and contained a GTEM cell located in a temperature controlled room (23°C) and a pulser that was triggered from a second copper mesh, RF-shielded enclosure.
Setup Microtiter plates were placed horizontally inside the GTEM cell.
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 20 kV/m - measured - 5-20 kV/m

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Low-dose UWB irradiation caused a mitogenic response in AML-12 mouse hepatocytes. During the post-exposure period of 8-24 h, a dose-dependent increase in cell viability was found in in both serum-treated and serum free medium -treated hepatocytes.
Cyclin A protein was induced in UWB exposed hepatocytes, suggesting that increased activity in the MTT assay after exposure was due to cell proliferation.
This study indicates that UWB irradiation has a mitogenic effect on AML-12 mouse hepatocytes.

Study character:

Study funded by

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