Study type: Medical/biological study (experimental study)

Exposure to ELF-pulse modulated X band microwaves increases in vitro human astrocytoma cell proliferation. med./bio.

Published in: Histol Histopathol 2009; 24 (12): 1551-1561

Aim of study (acc. to author)

To study whether exposure to microwave pulses in the X band range can induce cytotoxic effects on human astrocytoma cells.

Background/further details

Exposure experiments were replicated five times keeping cells in culture for 15, 30, 60 min and 24 h.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 9.6 GHz
Modulation type: AM, pulsed
Exposure duration: continuous for 15 min, 30 min, 60 min or 24 hr
Exposure 2: 9.6 GHz
Modulation type: AM, pulsed
Exposure duration: continuous for 15 min, 30 min, 60 min or 24 hr

Exposure 1

Main characteristics
Frequency 9.6 GHz
Type
Exposure duration continuous for 15 min, 30 min, 60 min or 24 hr
Modulation
Modulation type AM, pulsed
Modulation depth 90 %
Pulse width 100 ns
Rise time 1 ms
Repetition frequency 100 Hz
Exposure setup
Exposure source
Setup horn shaped GTEM cell equipped with anechoic walls, placed in a GTEM-incubator made of plastic material and heated by circulated water
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic field strength 3.3 mA/m peak value - - -
power 0.34 mW - - - -
electric field strength 1.25 V/m - - - -
SAR 0.4 mW/kg - calculated - -

Exposure 2

Main characteristics
Frequency 9.6 GHz
Type
Exposure duration continuous for 15 min, 30 min, 60 min or 24 hr
Modulation
Modulation type AM, pulsed
Modulation depth 90 %
Pulse width 120 ns
Rise time 1.25 ms
Repetition frequency 800 Hz
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic field strength 4.35 mA/m peak value - - -
power 0.6 mW - - - -
electric field strength 1.64 V/m - - - -
SAR 0.4 mW/kg - calculated - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data showed that cytoskeleton proteins, cell morphology and cell viability were not modified by exposure. A statistically significant increased cell proliferation rate was found under 24 h microwave exposure (both exposure experiments). An increased expression of connexin 43 proteins was found in exposed samples (24 h exposure, both experiments).

Study character:

Study funded by

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