To study whether exposure to microwave pulses in the X band range can induce cytotoxic effects on human astrocytoma cells.
Exposure experiments were replicated five times keeping cells in culture for 15, 30, 60 min and 24 h.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
9.6 GHz
Exposure duration:
continuous for 15 min, 30 min, 60 min or 24 hr
|
|
|
Exposure 2:
9.6 GHz
Exposure duration:
continuous for 15 min, 30 min, 60 min or 24 hr
|
|
| Frequency | 9.6 GHz |
|---|---|
| Type | |
| Exposure duration | continuous for 15 min, 30 min, 60 min or 24 hr |
| Exposure source | |
|---|---|
| Setup | horn shaped GTEM cell equipped with anechoic walls, placed in a GTEM-incubator made of plastic material and heated by circulated water |
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic field strength | 3.3 mA/m | peak value | - | - | - |
| power | 0.34 mW | - | - | - | - |
| electric field strength | 1.25 V/m | - | - | - | - |
| SAR | 0.4 mW/kg | - | calculated | - | - |
| Frequency | 9.6 GHz |
|---|---|
| Type | |
| Exposure duration | continuous for 15 min, 30 min, 60 min or 24 hr |
| Exposure source |
|
|---|---|
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic field strength | 4.35 mA/m | peak value | - | - | - |
| power | 0.6 mW | - | - | - | - |
| electric field strength | 1.64 V/m | - | - | - | - |
| SAR | 0.4 mW/kg | - | calculated | - | - |
The data showed that cytoskeleton proteins, cell morphology and cell viability were not modified by exposure. A statistically significant increased cell proliferation rate was found under 24 h microwave exposure (both exposure experiments). An increased expression of connexin 43 proteins was found in exposed samples (24 h exposure, both experiments).
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