Study type: Medical/biological study (experimental study)

Non-thermal microwave effects on protein dynamics? An X-ray diffraction study on tetragonal lysozyme crystals. med./bio.

Published in: Acta Crystallogr D Biol Crystallogr 2005; 61 Pt 2: 163-172

Aim of study (acc. to author)

To study the structural and dynamical effects of microwave fields on tetragonal single crystals of hen egg-white lysozyme.

Background/further details

Temperature controls were also performed.



Exposure Parameters
Exposure 1: 8 GHz
Exposure duration: continuous for 10 min and 4 h
  • power: 0.5 W unspecified (1 W, 2 W and 3 W)

Exposure 1

Main characteristics
Frequency 8 GHz
  • guided field
Exposure duration continuous for 10 min and 4 h
Modulation type unspecified
Exposure setup
Exposure source
Setup Crystals were located in a 1.25 mm wide gap between the central and the outer conductor where field strength was maximum. The salt solution reservoirs in each coil end of the capillary were located outside the slab-line, hence were not exposed to MMW.
Measurand Value Type Method Mass Remarks
power 0.5 W unspecified measured - 1 W, 2 W and 3 W

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • before exposure
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

High microwave power levels mainly led to increased, but largely recoverable, lattice defects owing to the evaporation of crystal water. At lower microwave power levels, the presence of the microwave field resulted in localized reproducible changes in the mean-square displacements (B factors).
The average B factor was found to increase with microwave power, consistent with an increase in temperature. However, the B factors of the atoms in a few highly localized sites in the protein showed reproducuble changes upon microwave exposure that are not explained by heating.

Study character:

Study funded by

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