Study type: Medical/biological study (experimental study)

Effects of 100 GHz radiation on alkaline phosphatase activity and antigen-antibody interaction. med./bio.

Published in: Bioelectromagnetics 2009; 30 (3): 167-175

Aim of study (acc. to author)

To study the effect of irradiation in the low frequency THz range (100 GHz) on two defined molecular interactions:
1) The interaction of soluble or immobilized alkaline phosphatase with the substrate p-nitrophenylphosphate and
2) the interaction between an antibody (mouse monoclonal anti-DNP) and its antigen (DNP).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 100 GHz
Exposure duration: continuous for 1 h or 2 h
Exposure 2: 100 GHz
Exposure duration: continuous for 1.5 h

Exposure 1

Main characteristics
Frequency 100 GHz
Type
Exposure duration continuous for 1 h or 2 h
Additional info exposure of AP
Exposure setup
Exposure source
Setup microplate with enzyme and substrate placed at the end of the waveguide tube inside an incubator
Parameters
Measurand Value Type Method Mass Remarks
power density 0.08 W/m² - measured - at the bottom of the microplate

Exposure 2

Main characteristics
Frequency 100 GHz
Type
Exposure duration continuous for 1.5 h
Additional info exposure of antigen and antibody
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
power density 0.08 W/m² - measured - at the bottom of the microplate

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

Exposure of enzyme either prior to addition of substrate or during the enzymatic reaction resulted in small but significant reductions in enzyme activity. These differences were not found if the enzyme had previously been immobilized onto plastic micro-wells.
Irradiation of immobilized antigen did not influence the ability of the antigen to interact with antibody. However, irradiation appeared to decrease the stability of previously formed antigen-antibody complexes.
The data suggest that 100 GHz exposure can induce small but statistically significant alterations in the characteristics of these two types of biomolecular interactions.

Study character:

Study funded by

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