Medical/biological study (experimental study, theoretical study)

Formulation of ELF magnetic fields' effects on malondialdehyde level and myeloperoxidase activity in kidney using genetic programming med./bio.

By: Tohumoglu G, Canseven AG, Cevik A, Seyhan N

Published in: Comput Methods Programs Biomed 2007; 86 (1): 1-9

Aim of study (acc. to author)

This study was designed to investigate whether effects of extremely low frequency magnetic fields on malondialdehyde level and myeloperoxidase activity can be appropiately analysed and formulated by a computer method.

Background/further details

A total of 54 male, 250-300 grams weighted, 10-12 weeks old, guiena pigs were used. Experimental results were compared to the results calculated by use of genetic programming (GP). Genetic programming belongs to evolutionary algorithms based on the principles of genetics and natural selections. It can be applid when biological effects are not measurable precisely as in this study the myeloperoxidase activity.

Endpoint

cell function: malondialdehyde level and myeloperoxidase activity

Exposure

- 50/60 Hz
- magnetic field

Exposure	Parameters
Exposure 1: 50 Hz Exposure duration: 4 h/day or 8 h/day for 5 days	magnetic flux density: 1 mT minimum magnetic flux density: 2 mT magnetic flux density: 3 mT maximum

Exposure 1

Main characteristics

Frequency	50 Hz
Type	magnetic field
Exposure duration	4 h/day or 8 h/day for 5 days

Exposure setup

Exposure source	Helmholtz coils
Setup	animals in 26 cm x 22 cm x 10 cm plastic cages in the center of the coil
Sham exposure	A sham exposure was conducted.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	1 mT	minimum	-	-	-
magnetic flux density	2 mT	-	-	-	-
magnetic flux density	3 mT	maximum	-	-	-

Exposed system:

animal
guinea pig
whole body

Methods Endpoint/measurement parameters/methodology

cell function: malondialdehyde level (spectrophotometry) and myeloperoxidase activity (assessment by measuring the H₂O₂-dependent oxidation of o-dianisidin): genetic programming

Investigated system:

isolated bio./chem. substance

Investigated organ system:

kidney

Time of investigation:

after exposure

Main outcome of study (acc. to author)

It was found that genetic programming showed high accuracy and can be therefore effectively used to model malondialdehyde level and myeloperoxidase activity.

Study character:

medical/biological study
experimental study
theoretical study
full/main study

Study funded by

not stated/no funding

Zhang Y et al. (2020): An Investigation Into the Effects of Long-Term 50-Hz Power-Frequency Electromagnetic Field Exposure on Hematogram, Blood Chemistry, Fibrosis, and Oxidant Stress Status in the Liver and the Kidney From Sprague-Dawley Rats
Erdal N et al. (2008): Effects of Long-term Exposure of Extremely Low Frequency Magnetic Field on Oxidative/Nitrosative Stress in Rat Liver
Eraslan G et al. (2007): Studies on antioxidant enzymes in mice exposed to pulsed electromagnetic fields
Jelenkovic A et al. (2006): Effects of extremely low-frequency magnetic field in the brain of rats
Akdag Z et al. (2006): Effect of ELF magnetic fields on lipid peroxidation, sperm count, p53, and trace elements
Seyhan N et al. (2006): In vivo effects of ELF MFs on collagen synthesis, free radical processes, natural antioxidant system, respiratory burst system, immune system activities, and electrolytes in the skin, plasma, spleen, lung, kidney, and brain tissues
Harakawa S et al. (2005): Effects of a 50 Hz electric field on plasma lipid peroxide level and antioxidant activity in rats
Regoli F et al. (2005): Pro-oxidant effects of extremely low frequency electromagnetic fields in the land snail Helix aspersa
Kula B et al. (2000): Effects of static and ELF magnetic fields on free-radical processes in rat liver and kidney