Study type: Medical/biological study (experimental study)

Exposure to extremely low frequency magnetic fields affects insulin-secreting cells. med./bio.

Published in: Bioelectromagnetics 2008; 29 (2): 118-124

Aim of study (acc. to author)

To evaluate the effects of extremely low frequency magnetic field on beta-cell survival and function, a insulin-secreting cell line, which exhibits responsiveness to glucose was cultured under sham exposure and extremely low frequency magnetic field conditions, and cell survival and function was assessed.

Background/further details

In diabetes, the loss of mass and function of beta cells, which are insulin-secreting components of pancreatic islets of Langerhans, is crucial.
Cells were cultured with 40 mg/dl and 100 mg/dl glucose.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: continuous for 2 days or 5 days

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration continuous for 2 days or 5 days
Exposure setup
Exposure source
Setup Helmholtz coil inside CO2 incubator, cylindrical exposure space with 20 cm diameter and a height of 14 cm, field vertical
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 5 mT - measured - -

Reference articles

  • Miyakoshi J et al. (1996): Increase in hypoxanthine-guanine phosphoribosyl transferase gene mutations by exposure to high-density 50-Hz magnetic fields.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Exposure to extremely low frequency magnetic field at 5 mT affected beta-cell survival and function; exposure at 5 mT for 5 days in the absence of glucose increased cell number, exposures for 2 days in the absence of glucose and for 5 days with 100 mg/dl glucose increased insulin secretion in the culture medium, and exposure for 2 and 5 days with 40 and 100 mg/dl glucose increased intracellular insulin concentration in HIT-T15 cells.
The increase in cell number under apoptotic culture conditions (absence of glucose) by exposure to extremely low frequency magnetic field could lead to new therapeutic concepts in the treatment of diabetes.

Study character:

Study funded by

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