|Pulse width||20 µs|
|Rise time||12 µs|
|Repetition frequency||50 Hz|
|electric field strength||7.1 V/m||maximum||estimated||-||-|
Pulsed electric fields produced a conformational change of insulin molecule. The binding capacity of insulin to its receptors was reduced to 87% of the control level after pulsed electric field exposure. The average intracellular tyrosine phosphorylation level decreased by 11%. The expression of 55 of 12000 genes investigated was modified, including an increase in the expression of human tyrosine phosphatase and the small GTP-binding protein.
Based on these data, a mechanism is proposed to explain the biological effects of pulsed electric fields on hepatocyte proliferation through the insulin signaling pathway.