Study type: Medical/biological study (experimental study)

Magnetic field anti-inflammatory effects in Crohn's disease depends upon viability and cytokine profile of the immune competent cells. med./bio.

Published in: J Physiol Pharmacol 2008; 59 (1): 177-187

Aim of study (acc. to author)

This study was performed to evaluate the effects of pulsating low frequency electromagnetic field on viability and cytokine production profile of human blood mononuclear cells involved in pathomechanism of the inflammatory bowel diseases.

Background/further details

Pulsating electromagnetic field treatment was studied in 8 patients (4 women, 4 men) with untreated newly diagnosed Crohn's disease (a chronic inflammatory bowel disease) and in 16 healthy controls (12 women and 4 men).
Peripherial blood cells were investigated mitogen (phytohaemagglutinin and lipopolysaccharides) stimulated, and not stimulated.

Endpoint

Exposure

Exposure Parameters
Exposure 1:
Modulation type: pulsed
Exposure duration: repeated daily exposure, 3 h/day, for 3 days, with 24-h intervals between exposures

Exposure 1

Main characteristics
Frequency
Type
Waveform
Exposure duration repeated daily exposure, 3 h/day, for 3 days, with 24-h intervals between exposures
Modulation
Modulation type pulsed
Repetition frequency 50 Hz
Exposure setup
Exposure source
  • generator
Setup A 96-well culture plate containing 0.2-ml aliquots of cells cultured for 48 h with or without mitogens was placed in the generator pocket inside the cell culture incubator.
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 45 mT - - - ± 5 mT

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Decreased cell viability was observed in exposed peripherial blood mononuclear cell cultures from both Crohn's disease patients and from healthy donors. The exposure was most effective after threefold application. Mitogen activated cells during cell division were most susceptible to induction of the cell death.
Decreased viability of the Crohn's disease derived cells upon magnetic field stimulation was accompanied by altered cytokines profile. Exposed and mitogen stimulated cells from Crohn's disease patients showed decreased IFN-gamma proinflammatory and increased IL-10 anti-inflammatory cytokine production.
The electromagnetically induced cell death could be an important step for non-invasive pulsed electromagnetic field treatment in chronic inflammatory diseases.

Study character:

Study funded by

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