This study was performed to evaluate the effects of pulsating low frequency electromagnetic field on viability and cytokine production profile of human blood mononuclear cells involved in pathomechanism of the inflammatory bowel diseases.
Pulsating electromagnetic field treatment was studied in 8 patients (4 women, 4 men) with untreated newly diagnosed Crohn's disease (a chronic inflammatory bowel disease) and in 16 healthy controls (12 women and 4 men).
Peripherial blood cells were investigated mitogen (phytohaemagglutinin and lipopolysaccharides) stimulated, and not stimulated.
|Repetition frequency||50 Hz|
|magnetic flux density||45 mT||-||-||-||± 5 mT|
Decreased cell viability was observed in exposed peripherial blood mononuclear cell cultures from both Crohn's disease patients and from healthy donors. The exposure was most effective after threefold application. Mitogen activated cells during cell division were most susceptible to induction of the cell death.
Decreased viability of the Crohn's disease derived cells upon magnetic field stimulation was accompanied by altered cytokines profile. Exposed and mitogen stimulated cells from Crohn's disease patients showed decreased IFN-gamma proinflammatory and increased IL-10 anti-inflammatory cytokine production.
The electromagnetically induced cell death could be an important step for non-invasive pulsed electromagnetic field treatment in chronic inflammatory diseases.