Study type: Medical/biological study (experimental study)

Lung cancer A549 cells migrate directionally in DC electric fields with polarized and activated EGFRs. med./bio.

Published in: Bioelectromagnetics 2009; 30 (1): 29-35

Aim of study (acc. to editor)

To study the effect of DC electric fields on migration direction (galvanotaxis) of lung cancer cells, and the role of epidermal growth factor receptors (EGFR).

Background/further details

The EGFR inhibitors cetuximab and AG1478 were used to test the possible involvement of EGFR activity in galvanotactic reaction. To explore the mechanism through which DC electric fields stimulate the migration of A549 cells, the authors investigated the involvement of EGFR downstream signaling pathways ERK and AKT.

Endpoint

Exposure

Exposure Parameters
Exposure 1:
Exposure duration: continuous for 2 hours

Exposure 1

Main characteristics
Frequency
Type
Exposure duration continuous for 2 hours
Exposure setup
Exposure source
Chamber 32 mm x 8 mm x 0.12 mm glass chamber or 60 mm x 15 mm x 0.12 mm glass chamber
Setup silver/silver chloride electrodes
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 3 V/cm - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • before exposure
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

A549 cells migrated toward the cathode in applied DC electric fields at 3 V/cm. Both epidermal growth factor receptors and F-actin are polarized to the cathode (i.e. stronger staining of EGFRs at the cathodal side of the plasma membrane). EGFR inhibitors (cetuximab and AG1478) reduced the migration rate and directed motility in DC electric fields. ERK and AKT signaling pathways were prominently promoted by the electric field. EGFR inhibitors could reduce this promotion but not completely.
These findings suggest that polarization of EGFRs and the activation of their downstream signals play an important role in the galvanotaxis of A549 cells in DC electric fields.

Study character:

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