To assess the effects of terahertz irradiation on human skin by assessing the effects on the primary keratinocytes ability to continue growth and normal patterns of differentiation.
Primary human keratinocytes were exposed to different terahertz sources with different durations.
| Frequency | 0.2–3 THz |
|---|---|
| Type | |
| Exposure duration | continuous for 10, 20, or 30 min |
| Modulation type | pulsed |
|---|---|
| Pulse width | 2 ps |
| Repetition frequency | 80 MHz |
| Additional info |
total pulse duration of 20-30 ps with about 90% of the power delivered within the first 2 ps |
| Exposure source |
|
|---|---|
| Chamber | A Ti:Sapphire laser was used impacting on an electro-optic photoconverter. |
| Setup | The keratinocytes were seeded into a 96-well tissue culture plate at 100 µl per well. They were exposed through the base of the 96-well plate. |
| Sham exposure | A sham exposure was conducted. |
| Additional info | Two sets of 4 control wells were exposed to the same conditions but not to the THz beam. |
| Frequency | 0.1–2.7 THz |
|---|---|
| Type | |
| Exposure duration | continuous for 10, 20, or 30 min |
| Modulation type | pulsed |
|---|---|
| Pulse width | 0.25 ps |
| Repetition frequency | 250 kHz |
| Exposure source |
|
|---|---|
| Chamber | A Ti:Sapphire laser (800 nm) was used impacting on a gallium arsenide wide aperture antenna with a large DC-bias applied across the device. The radiation was collected, collimated, and focused by two parabolae with a spot size of 130 µm to 3.7 mm. |
| Sham exposure | A sham exposure was conducted. |
There were no significant effects following terahertz exposure on the human primary keratinocyte redox potential, compared with the control groups. The differentiation occured in a normal way for exposed and control groups. The fluorescein cadaverine incorporation increased between day 3 and day 8 after exposure.
The results from the different terahertz exposures showed no signs of induced stress to cells.
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