Study type: Medical/biological study (experimental study)

Terahertz radiation increases genomic instability in human lymphocytes. med./bio.

Published in: Radiat Res 2008; 170 (2): 224-234

Aim of study (acc. to author)

To explore the genetic and epigenetic consequences of exposing dividing human peripheral lymphocytes to low-intensity continuous wave 0.1 THz irradiation. Aneuploidy of the chromosomes as well as replication timing and synchronization of the centromeres were examined.

Background/further details

Increased levels of aneuploidy and asynchronous replication are typical of both hematological malignancies and various solid tumors. Lymphocytes were obtained from nine male volunteers. Three groups were examined: a control group that was undisturbed for the duration of the culturing, a sham-exposed group and an exposed sample. To ascertain that the effects induced by exposing the cells were not thermal, temperature rise was monitored during exposure.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 0.1 THz
Modulation type: CW
Exposure duration: continuous for 1 h, 2 h or 24 h

Exposure 1

Main characteristics
Frequency 0.1 THz
Type
Polarization
Exposure duration continuous for 1 h, 2 h or 24 h
Modulation
Modulation type CW
Exposure setup
Exposure source
Distance between exposed object and exposure source 28 cm
Setup 33.4 cm long waveguide with an inner diameter of 3.8 cm placed inside a temperature controlled incubator; inner walls of incubator covered with pyramidal radiation-absorbing material
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
power density 0.031 mW/cm² - calculated - -
SAR 2.4 mW/g - calculated - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The present study suggests the existence of genetic instability, reflected by increased levels of aneuploidy and increased levels of asynchronous replication of centromeres, in lymphocytes due to exposure to 0.1 THz continous wave radiation. Chromosomes 11 and 17 were most vulnerable (about 30% increase in aneuploidy after 2 and 24 h of exposure) due to exposure, while chromosomes 1 and 10 were not affected. There were changes in the asynchronous mode of replication of centromeres 11, 17 and 1 (by 40%) after 2 h of exposure and in the centromeres of all four chromosomes after 24 h of exposure (by 50%).

Study character:

Study funded by

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