To explore the genetic and epigenetic consequences of exposing dividing human peripheral lymphocytes to low-intensity continuous wave 0.1 THz irradiation. Aneuploidy of the chromosomes as well as replication timing and synchronization of the centromeres were examined.
Increased levels of aneuploidy and asynchronous replication are typical of both hematological malignancies and various solid tumors. Lymphocytes were obtained from nine male volunteers. Three groups were examined: a control group that was undisturbed for the duration of the culturing, a sham-exposed group and an exposed sample. To ascertain that the effects induced by exposing the cells were not thermal, temperature rise was monitored during exposure.
|Distance between exposed object and exposure source||28 cm|
|Setup||33.4 cm long waveguide with an inner diameter of 3.8 cm placed inside a temperature controlled incubator; inner walls of incubator covered with pyramidal radiation-absorbing material|
|Sham exposure||A sham exposure was conducted.|
The present study suggests the existence of genetic instability, reflected by increased levels of aneuploidy and increased levels of asynchronous replication of centromeres, in lymphocytes due to exposure to 0.1 THz continous wave radiation. Chromosomes 11 and 17 were most vulnerable (about 30% increase in aneuploidy after 2 and 24 h of exposure) due to exposure, while chromosomes 1 and 10 were not affected. There were changes in the asynchronous mode of replication of centromeres 11, 17 and 1 (by 40%) after 2 h of exposure and in the centromeres of all four chromosomes after 24 h of exposure (by 50%).