Study type: Medical/biological study (experimental study)

Millimeter-wave exposure promotes the differentiation of bone marrow stromal cells into cells with a neural phenotype. med./bio.

Published in: J Huazhong Univ Sci Technolog Med Sci 2009; 29 (4): 409-412

Aim of study (acc. to author)

The study investigated the ability of millimeter waves to promote the differentiation of rat primary bone marrow stromal cells into cells with a neural phenotype, in an attempt to provide experimental basis for the future clinical application.

Background/further details

At cell passage 3, the cells were treated by beta-mercaptoethanol (to induce cell differentiation) alone or in combination with millimeter waves.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 36.11 GHz
Exposure duration: 10 minutes, three times at an interval of 2 h

General information

cells induced by i) beta-mercaptoethanol (BME) ii) BME + millimeter waves iii) control group

Exposure 1

Main characteristics
Frequency 36.11 GHz
Type
Exposure duration 10 minutes, three times at an interval of 2 h
Exposure setup
Exposure source
Setup culture plate placed under the radiator in a thermotank at 37 °C
Parameters
Measurand Value Type Method Mass Remarks
power density 10 mW/cm² - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated material:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The untreated bone marrow stromal cells predominately expressed nucleostemin (a marker protein of stem cells prior to differentiation). After treatment by beta-mercaptoethanol and millimeter waves, the cells exhibited a dramatic decrease in nucleostemin expression and increase in neuron-specific enolase expression (a marker protein for neurons). The differentiation rate of the cells treated with beta-mercaptoethanol and millimeter wave exposure in combination was significantly higher than that of the cells treated with beta-mercaptoethanol alone.
The authors conclude that millimeter wave exposure enhanced the inducing effect of beta-mercaptoethanol on the differentiation of bone marrow stromal cells into cells with a neural phenotype.

Study character:

Study funded by

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