Study type: Medical/biological study (experimental study)

In vitro investigation of the biological effects associated with human dermal fibroblasts exposed to 2.52 THz radiation. med./bio.

Published in: Lasers Surg Med 2011; 43 (2): 152-163

Aim of study (acc. to author)

To investigate the cellular and molecular response of human dermal fibroblasts to exposed to 2.52 THz exposure.

Background/further details

Firstly, the viability of THz-exposed cells was compared to hyperthermic positive controls (40 °C). Additionally, to determine protein and/or DNA damage, the protein expression and gene expression was determined in THz-exposed cells in comparison to hyperthermic positive controls (40 °C) and genotoxic positive controls (UV exposure).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 2.52 THz
Exposure duration: continuous for 5 min., 10 min., 20 min., 40 min., 80 min.

General information

cells were treated i) with THz radiation ii) with heat shock at 40° C for 5, 10, 20, 40, 80 min. (positive control) iii) with UV exposure ( λ = 254 nm, P = 38 W) for 3 min. (positive control

Exposure 1

Main characteristics
Frequency 2.52 THz
Type
Exposure duration continuous for 5 min., 10 min., 20 min., 40 min., 80 min.
Exposure setup
Exposure source
Setup THz laser beam radius = 3 mm
Parameters
Measurand Value Type Method Mass Remarks
power 24 mW - calculated - -
power density 84.4 mW/cm² - calculated - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The cellular temperatures increased by 3 °C during all THz exposures. The THz and hyperthermic exposure groups exhibited equivalent viability (levels of cell survival of 90 %) and heat shock protein expression (3.5-fold increases) for each exposure duration tested. The gene expression was unchanged in both groups; however, appreciable increases were observed in the genotoxic positive control group.
The results show that the exposure to 2.52 THz radiation generates primarily thermal effects in human dermal fibroblasts. The authors conclude that THz-induced effects may be accurately predicted with conventional thermal damage models.

Study character:

Study funded by

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