Study type: Medical/biological study (experimental study)

The expression and intranuclear distribution of nucleolin in HL-60 and K-562 cells after repeated, short-term exposition to rotating magnetic fields. med./bio.

Published in: Int J Radiat Biol 2008; 84 (9): 752-760

Aim of study (acc. to author)

To study the influence of rotating magnetic fields on the expression and intra-nuclear distribution of nucleolin (a protein involved e.g. in ribosome biosynthesis) in two human cell lines.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 35 Hz
Exposure duration: 30 min with an interval of 24 h on 4 days
Exposure 2: 50 Hz
Exposure duration: 30 min with an interval of 24 h on 4 days

Exposure 1

Main characteristics
Frequency 35 Hz
Type
Exposure duration 30 min with an interval of 24 h on 4 days
Additional info rotating field
Exposure setup
Exposure source
  • generator made of the stator of a 3-phase induction squirrel-cage motor
Setup water-filled glass container with the cultur flasks placed inside the stator
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 10 mT mean calculated - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Exposure duration 30 min with an interval of 24 h on 4 days
Additional info rotating field
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 20 mT mean calculated - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

In both cell lines repeated short-term exposure to rotating magnetic fields induced significant increase in nucleolin expression in the whole nucleus and in nucleolin aggregates. This effect seemed to be frequency-dependent for K-562 cells while the increase of nucleolin expression in HL-60 cells was higher at the lower frequency. No significant changes in cell cycle phases in both cell lines after exposure were found. This indicates that changes of nucleolin expression induced by rotating magnetic fields are not related to cell cycle.
In conclusion, the nucleolin is responsive to rotating magnetic fields in HL-60 and K-562. The increase of its expression may indicate a reaction of cells to rotating magnetic fields and it may influence other biological properties.

Study character:

Study funded by

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