Study type: Medical/biological study (experimental study)

Alterations in the cell cycle and in the protein level of cyclin D1, p21CIP1, and p16INK4a after exposure to 50 Hz MF in human cells. med./bio.

Published in: Radiat Environ Biophys 2002; 41 (2): 131-137

Aim of study (acc. to author)

To investigate the effects of magnetic field exposure on cell cycle, DNA synthesis in the S-phase, and some selected G1-phase regulating proteins were investigated in human amniotic fluid cells.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 24 h
Exposure 2: 50 Hz
Exposure duration: continuous for 30 h

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 24 h
Exposure setup
Exposure source
Chamber CO2 incubator
Setup Cells were placed in the central area of the coils where the magnetic field was uniform
Additional info Control cultures were kept in an identical incubator without the Helmholtz coils
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - measured - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 30 h
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The magnetic field exposure leads to a significant reduction of S-phase cells. The Cdk4 protein level was not altered. Cyclin D1 protein level was decreased after a MF-exposure of 24 h and 30 h. The protein levels of p16INK4a and p21CIP1, both are Cdk inhibitors, were found to be increased after 1 h and 12 h and after 6 h and 12 h, respectively. However, both Cdk inhibitor levels were reduced after longer exposure times of 24 h and 30 h.
The results of this study suggest an inhibitory effect of magnetic field exposure on the G1-phase of the cell cycle of human amniotic fluid cells.

Study character:

Study funded by

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