Study type: Medical/biological study (experimental study)

Enhancement of the hydrolysis activity of F0F1-ATPases using 60 Hz magnetic fields. med./bio.

Published in: Bioelectromagnetics 2009; 30 (8): 663-668

Aim of study (acc. to author)

To study the effects of extremely low frequency electromagnetic fields on membrane F0F1-ATPase enzyme activity in chromatophore suspensions (as a possible initial step in a cascade of events that could affect health).

Background/further details

F0F1-ATPase is composed of two parts, membrane-embedded part F0 and hydrophilic catalytic part F1. F0F1-ATPase interconverts two major "energy currencies" (the transmembrane electrochemical potential difference of protons and ATP).
F0F1-ATPases were also treated with N,N-dicyclohexylcarbodiimide (DCCD, for inhibition of subunit F0) and lithium chloride (also for hydrolysis inhibition). Additionally, the chromatophores were labeled with the pH indicator F-DHPE (proton transfer experiment (function of F0 is the proton transfer)).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: continuous for 20 min

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration continuous for 20 min
Exposure setup
Exposure source
Setup two 12 cm x 6 cm rectangular coils; each coil consisting of two sub-coils with 100 turns of 1 mm copper wire each, separated by 7 cm; non-uniformity of the magnetic field in the exposue aerea < 2%
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.1 mT minimum measured - -
magnetic flux density 0.5 mT maximum measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

60 Hz magnetic fields enhanced the enzyme activity of F0F1-ATPase. The effects depended on magnetic intensity: 0.3 and 0.5 mT magnetic fields enhanced the hydrolysis activity, whereas 0.1 mT exposure caused no significant changes. The magnetic fields mainly affected the subunit F1: even if the F0F1-ATPase was inhibited by DCCD (inhibition of F0), its hydrolysis activity was enhanced by a 0.5 mT magnetic field. Moreover, when the F-DHPE-labeled chromatophores were exposed to 0.5 mT, it was found that the pH of the outer membrane of the chromatophore was unchanged.
The data may provide a new way to explore a relationship between the effects of magnetic fields on human health and the effects of magnetic fields on membrane F0F1-ATPase (F1 may be the target affected by magnetic fields).

Study character:

Study funded by

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