For 31P NMR spectroscopy 20 snails were exposed (10 snails for seven days and 10 snails for 16 days) and eight snails were sham exposed for seven days. For biochemical analysis three snails were exposed for seven days and four snails for 16 days (the control group consisted of five snails).
Exposure duration: continuous for 7 or 16 days
|Distance between exposed object and exposure source||20 cm|
|Setup||solenoid with a regular laminated transformer core and pole dimensions of 9.5 cm x 9.5 cm used as electromagnet; snails were placed in a 26 cm x 43 cm x 15 cm polycarbonate box in front of the magnet|
|Sham exposure||A sham exposure was conducted.|
|magnetic flux density||0.5 mT||average over time||measured||-||at the middle of the box|
Exposure of snails to the extremely low frequency magnetic field for a period of seven days shifted intracellular pH toward more alkaline conditions, and increased the activity of investigated enzymes involved in phosphate turnover. Prolonged exposure for the period of 16 days caused a decrease of phosphocreatine and ATP levels and decreased enzyme activities, compared to the seven day exposure.
The authors conclude that exposure to the extremely low frequency magnetic field for a period of seven days increased the overall phosphate turnover in the snail nervous system, while after prolonged exposure phosphate metabolism adopt to the exposure.