Study type: Medical/biological study (experimental study)

Magnetic Fields at Extremely Low-Frequency (50 Hz, 0.8 mT) Can Induce the Uptake of Intracellular Calcium Levels in Osteoblasts. med./bio.

Published in: Biochem Biophys Res Commun 2010; 396 (3): 662-666

Aim of study (acc. to author)

Rat osteoblasts were used as a model to test the hypothesis that extremely low frequency magnetic fields can alter the concentrations of intracellular calcium, and to examine if there is a window effect.

Background/further details

10 or more cells were monitored simultaneously for up to 10 min.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 15–100 Hz
Modulation type: pulsed
Exposure duration: continuous for 9 min. (90 s for each frequency)

Exposure 1

Main characteristics
Frequency 15–100 Hz
Type
Exposure duration continuous for 9 min. (90 s for each frequency)
Additional info the following frequencies were applied: 15 Hz, 20 Hz, 50 Hz, 60 Hz, 75 Hz, 100 Hz
Modulation
Modulation type pulsed
Duty cycle 7 %
Exposure setup
Exposure source
Setup pair of Helmholtz coils mounted on the objective stage of an inverted fluorescence microscope; coils with a diameter of 8 cm, made of 185 turns of 0.9 mm enameled copper wire; distance between the coils = 4 cm; cells placed between the coils
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.1 mT - measured - -
magnetic flux density 0.5 mT - measured - -
magnetic flux density 0.8 mT - measured - -
magnetic flux density 1.1 mT - measured - -
magnetic flux density 1.4 mT - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • before exposure
  • during exposure

Main outcome of study (acc. to author)

The data showed that a 50 Hz, 0.8 mT magnetic field can induce the uptake of calcium in osteoblasts (window effect).

Study character:

Study funded by

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