Cells were divided into the following groups: exposure to a magnetic field with 1) 0.6 mT, 2) 1.2 mT, 3) 1.8 mT, 4) 2.4 mT, 5) 3.0 mT, 6) 3.6 mT and 7) sham exposure. Additionally, cells with a block in primary cilium formation were created via transfection by siRNA and a resulting suppressed expression of the intraflagellar transport (IFT) protein 88. These cells were exposed to a 0,6 mT magnetic field (group 8). Each experiment was conducted with n=3 samples per group and was repeated 3 times.
solenoid (27 cm long, three series-connected coils and lead tube core cylinder with inner diameter of 100 mm) was placed in a CO2incubator (5% CO2, 37 °C and 100% humidity); fields had an error of <0.1% horizontally (from 60 to -60 mm) and <0.05% vertically (from 45 to -45 mm)
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(2019):
Sinusoidal Electromagnetic Fields Increase Peak Bone Mass in Rats by Activating Wnt10b/β-Catenin in Primary Cilia of Osteoblasts
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Pulsed electromagnetic fields stimulate osteogenic differentiation and maturation of osteoblasts by upregulating the expression of BMPRII localized at the base of primary cilium
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Magnetic Fields at Extremely Low-Frequency (50 Hz, 0.8 mT) Can Induce the Uptake of Intracellular Calcium Levels in Osteoblasts
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(2010):
EMF acts on rat bone marrow mesenchymal stem cells to promote differentiation to osteoblasts and to inhibit differentiation to adipocytes
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Pulsed electromagnetic fields enhance BMP-2 dependent osteoblastic differentiation of human mesenchymal stem cells
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Effects of different extremely low-frequency electromagnetic fields on osteoblasts
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Effects of electromagnetic fields on cells: physiological and therapeutical approaches and molecular mechanisms of interaction. A review
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Effect of electromagnetic fields on proliferation and differentiation of cultured mouse bone marrow mesenchymal stem cells
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