Study type: Medical/biological study (experimental study)

Subchronic exposure of hsp70.1-deficient mice to radiofrequency radiation. med./bio.

Published in: Int J Radiat Biol 2005; 81 (10): 781-792

Aim of study (acc. to author)

To determine whether sub-chronic radiofrequency exposure can cause constitutive induction of a stress response at a cellular and/or molecular level in hsp70.1-deficient mice due to repeated stimulation.

Background/further details

Previously the authors generated hsp70.1-deficient mice to elucidate the in vivo function of HSP70 (heat shock protein 70) in detail. The renal tissues and embryonic fibroblasts of these mice were shown to be more vulnerable to hyperosmotic stress.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 849 MHz
Exposure duration: twice daily for 45 min with a 15 min interval, 5 days a week for 4, 8 and 10 weeks
Exposure 2: 849 MHz
Exposure duration: one or two 45 min exposures
Exposure 3: 1,763 MHz
Exposure duration: twice daily for 45 min with a 15 min interval, 5 days a week for 4, 8 and 10 weeks
Exposure 4: 1,763 MHz
Exposure duration: one or two 45 min exposures

Exposure 1

Main characteristics
Frequency 849 MHz
Type
Exposure duration twice daily for 45 min with a 15 min interval, 5 days a week for 4, 8 and 10 weeks
Exposure setup
Exposure source
Chamber Stainless steel reverberation chamber (2293 mm x 2296 mm x 1700 mm) fitted with mode stirrer for field uniformity
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
SAR 0.4 W/kg mean calculated whole body -
power 60 W maximum - - -

Exposure 2

Main characteristics
Frequency 849 MHz
Type
Exposure duration one or two 45 min exposures
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
SAR 0.4 W/kg mean calculated whole body -

Exposure 3

Main characteristics
Frequency 1,763 MHz
Type
Exposure duration twice daily for 45 min with a 15 min interval, 5 days a week for 4, 8 and 10 weeks
Exposure setup
Exposure source
  • not specified
Parameters
Measurand Value Type Method Mass Remarks
SAR 0.4 W/kg mean calculated whole body -

Exposure 4

Main characteristics
Frequency 1,763 MHz
Type
Exposure duration one or two 45 min exposures
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
SAR 0.4 W/kg mean calculated whole body -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No difference was revealed in the histopathological analysis between sham-exposed and radiofrequency exposed mice. There was no evidence of increased proliferative and apoptotic activities.
The levels of the heat shock proteins showed no obvious changes. Radiofrequency irradiation did not affect the phosphorylation status of stress-activated kinases.
The hsp70.1-deficient mice did not show any significant changes in terms of cell proliferation, apoptosis, or stress response due to exposure of 849 MHz or 1763 MHz radiofrequency fields.

Study character:

Study funded by

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