Study type: Medical/biological study (experimental study)

Effect of 60 Hz magnetic fields on the activation of hsp70 promoter in cultured INER-37 and RMA E7 cells. med./bio.

Published in: In Vitro Cell Dev Biol Anim 2010; 46 (9): 758-763

Aim of study (acc. to author)

To study the effect of a 60 Hz electromagnetic field on the activity of hsp70 promoter in cells transfected with the luciferase gene as reporter gene.

Background/further details

Experiments were carried out in five groups (for each cell line): 1) non-transfected cells as control (without magnetic field exposure or thermal treatment), 2) transfected cells but not exposed to the magnetic field or thermal treatment, 3) transfected cells treated with a thermal shock (43°C), 4) transfected cells exposed to the magnetic field at 8 µT, and 5) transfected cells exposed to the magnetic field at 80 µT. Five cell cultures were used for each treatment regimen and controls.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: continuous for 20 min

General information

cells were treated with i) 8 µT EMF ii) 80 µT EMF iii) 43° C heat shock

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration continuous for 20 min
Exposure setup
Exposure source
Setup 25 cm long solenoid with a radius of 5.27 cm and 340 turns of enamel isolated copper wire with a diameter of 1.3 mm placed inside a temperature-controlled incubator; cultures positioned in the middle of the solenoid where the magnetic field was homogeneous
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 8 µT effective value measured - -
magnetic flux density 80 µT effective value measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data showed an increased luciferase gene expression in INER-37 cells exposed to the magnetic field or thermal shock compared with controls, but the magnetic field exposure had no effect on the RMA E7 cell line.

Study character:

Study funded by

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