Experiments were carried out in five groups (for each cell line): 1) non-transfected cells as control (without magnetic field exposure or thermal treatment), 2) transfected cells but not exposed to the magnetic field or thermal treatment, 3) transfected cells treated with a thermal shock (43°C), 4) transfected cells exposed to the magnetic field at 8 µT, and 5) transfected cells exposed to the magnetic field at 80 µT. Five cell cultures were used for each treatment regimen and controls.
|Setup||25 cm long solenoid with a radius of 5.27 cm and 340 turns of enamel isolated copper wire with a diameter of 1.3 mm placed inside a temperature-controlled incubator; cultures positioned in the middle of the solenoid where the magnetic field was homogeneous|
|Sham exposure||A sham exposure was conducted.|
The data showed an increased luciferase gene expression in INER-37 cells exposed to the magnetic field or thermal shock compared with controls, but the magnetic field exposure had no effect on the RMA E7 cell line.