Study type: Medical/biological study (experimental study)

Effect of 50 Hz electromagnetic fields on the induction of heat-shock protein gene expression in human leukocytes. med./bio.

Published in: Radiat Res 2004; 161 (4): 430-434

Aim of study (acc. to author)

To determine whether exposure to power-frequency magnetic fields in the range 0-100 µT either alone or concomitant with mild heating induced heat shock protein gene expression in human leukocytes. This response was compared to that induced by heat alone.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 4 h

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 4 h
Exposure setup
Exposure source
Chamber Wooden incubator at 37°C.
Setup three coils with 0.25 m diameter, 0.15 m apart, stacked vertically; six 96-well plate holders were stacked in an open frame, vertically above each other between the coils; 16 samples were placed in a cluster of wells at the center of each plate.
Additional info Additional samples were maintained in a separate incubator at 37°C for the same period. A subset was heat shocked at 42°C for the final 2 h of the 4 h experimental period which served as positive control.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 20 µT minimum measured - -
magnetic flux density 40 µT - measured - -
magnetic flux density 60 µT - measured - -
magnetic flux density 80 µT - measured - -
magnetic flux density 100 µT maximum measured - -

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No evidence was found that 50 Hz power frequency magnetic fields of amplitudes 0-100 µT induce expression of genes encoding heat shock proteins in human peripheral blood leukocytes. Additionally, exposure to mild heat at 40°C did not sensitize the cells to a concomitant exposure to power-frequency magnetic fields.

Study character:

Study funded by

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