To determine whether exposure to power-frequency magnetic fields in the range 0-100 µT either alone or concomitant with mild heating induced heat shock protein gene expression in human leukocytes. This response was compared to that induced by heat alone.
|Chamber||Wooden incubator at 37°C.|
|Setup||three coils with 0.25 m diameter, 0.15 m apart, stacked vertically; six 96-well plate holders were stacked in an open frame, vertically above each other between the coils; 16 samples were placed in a cluster of wells at the center of each plate.|
|Additional info||Additional samples were maintained in a separate incubator at 37°C for the same period. A subset was heat shocked at 42°C for the final 2 h of the 4 h experimental period which served as positive control.|
No evidence was found that 50 Hz power frequency magnetic fields of amplitudes 0-100 µT induce expression of genes encoding heat shock proteins in human peripheral blood leukocytes. Additionally, exposure to mild heat at 40°C did not sensitize the cells to a concomitant exposure to power-frequency magnetic fields.