Study type: Medical/biological study (experimental study)

Effects of 50 Hz sinusoidal magnetic fields on Hsp27, Hsp70, Hsp90 expression in porcine aortic endothelial cells (PAEC). med./bio.

Published in: Bioelectromagnetics 2007; 28 (3): 231-237

Aim of study (acc. to author)

To study the influence of 50 Hz sinusoidal magnetic field on Hsp27, Hsp70, and Hsp90 expression in a model of primary culture of porcine aortic endothelial cells.

Background/further details

Endothelial cells are involved in the onset of many diseases.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 4 h

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 4 h
Exposure setup
Exposure source
  • coils were wound by a pair of parallel wires, outer coils 42 turns and inner coils 37 turns
Chamber Both sham and exposure coil systems were placed in the same incubator. A µ-metal foil was placed between the two systems resulting in 30% reduction of the magnetic field.
Setup Four coaxial coils placed horizontally and thus parallel with respect to the surface of the culture well plates. The diameter of the external coils (top and bottom) was 18 cm, while that of the internal ones was 20 cm. The geometry of the coil system was calculated to optimize the extension of the magnetic field uniformity. the distances between the external and internal coils from the center of the symmetry were 9 and 3 cm, respectively. According to these conditions the uniformity was better than 2% within a cylindrical region of ca. 11 cm (along the coil axis) x 9 cm, thus allowing simultaneous exposure of 6 culture well plates.
Sham exposure A sham exposure was conducted.
Additional info Sham field conditions (5 µT) were achieved by configuring the coils in counter wound arrangement to cancel each others magnetic field.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT effective value measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Extremely low frequency magnetic field exposure induced an increase in the mRNA levels of the three proteins, which was statistically significant for Hsp70. On the contrary, no influence on Hsp27, Hsp70, and Hsp90 protein levels was observed. The magnetic field exposure affected the cellular distribution of Hsp27; in particular a partial relocalization in the nucleus was found.

Study character:

Study funded by

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