Study type: Medical/biological study (experimental study)

Microwave induces apoptosis in A549 human lung carcinoma cell line. med./bio.

Published in: Chin Med J (Engl) 2011; 124 (8): 1193-1198

Aim of study (acc. to author)

To study the effects of microwave irradiation on damaging changes and apoptosis indexes in A549 lung cancer cell line (for a therapeutical approach in lung cancer treatment).

Endpoint

Exposure

Exposure Parameters
Exposure 1:
  • unspecified
Exposure duration: continuous for 20 min
Exposure 2:
  • unspecified
Exposure duration: continuous for 10 min
Exposure 3:
  • unspecified
Exposure duration: continuous for 30 min

Exposure 1

Main characteristics
Frequency
  • unspecified
Type
Exposure duration continuous for 20 min
Exposure setup
Exposure source
Setup cells exposed in culture plates with 96 wells (200 µl/well) or 6 wells (1 ml/well)
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
power density 200 mW/cm² - - - group A
power density 400 mW/cm² - - - group B
power density 600 mW/cm² - - - group C

Exposure 2

Main characteristics
Frequency
  • unspecified
Type
Exposure duration continuous for 10 min
Exposure setup
Exposure source
Setup cells exposed in culture plates with 96 wells (200 µl/well) or 6 wells (1 ml/well)
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
power density 600 mW/cm² - - - group D

Exposure 3

Main characteristics
Frequency
  • unspecified
Type
Exposure duration continuous for 30 min
Exposure setup
Exposure source
Setup cells exposed in culture plates with 96 wells (200 µl/well) or 6 wells (1 ml/well)
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
power density 600 mW/cm² - - - group E

Exposed system:

Methods Endpoint/measurement parameters/methodology

Main outcome of study (acc. to author)

Microwave exposure caused morphological changes in the A549 cells, including cell shrinking and nuclear pyknosis. Microwave exposure also significantly inhibited metabolic activities and induced apoptosis in A549 cells: The results of the MTT assay showed a significant decrease of cell activities in all exposure groups compared with the control group in a time-dependent manner and to some extent in a dose-depenent manner. Apoptosis rate was increased in all exposed groups compared to the control group in a time- and dose-dependent manner. Downregulation of Bcl-2 protein expression and upregulation of p53 expression were observed after exposure.
In concludion, microwaves can promote apoptosis in A549 cells. The effect depends on the duration and dosage of the microwave exposure. Bcl-2 and p53 proteins may be involved in the apoptotic process of A549 cells induced by microwaves. The data imply the possibility that microwaves might provide a novel approach in lung cancer treatment.

Study character:

Study funded by

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